C6788

4-Chloro-1-naphthol

peroxidase substrate, tablet, suitable for use in immunoblotting procedures

• Synonym(s): 4-CN, 4-chloronaphthalen-1-ol
• Linear Formula: ClC₁₀H₆OH
• CAS Number: 604-44-4
• Molecular Weight: 178.61
• Beilstein: 509750
• EC Number: 210-068-5
• MDL number: MFCD00003974
• UNSPSC Code: 12352204
• PubChem Substance ID: 57654035
• NACRES: NA.83

Properties

• product name: 4-Chloro-1-naphthol, tablet
• form: tablet
• mp: 118-121 °C (lit.)
• solubility: methanol: 10 mL, clear, colorless
• storage temp.: −20°C
• SMILES string: Oc1ccc(Cl)c2ccccc12
• InChI: 1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H
• InChI key: LVSPDZAGCBEQAV-UHFFFAOYSA-N

Description

Specificity

4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.

Application

4-Chloro-1-naphthol has been used as a substrate in enzyme-linked immunospot (ELISpot) assay for detection of the spots. It has also been used as a precipitating chromogenic substrate for visualizing the antigen−antibody-reactive bands following western blotting.

Quantity

Contains 30 mg substrate per tablet.

Reconstitution

Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves