Conjugating PEG to catalase is recognized to improve protein stability, leading to an extended circulatory half-life for the enzyme.
C4963
Catalase−polyethylene glycol
lyophilized powder, ~40,000 units/mg protein
Synonym(s):
PEG-Catalase
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2 MG
MXP 3,903.00
About This Item
Recommended Products
form
lyophilized powder
Quality Level
specific activity
~40,000 units/mg protein
mol wt
PEG 5,000
composition
Protein, ~50% E405
extent of labeling
~40 mol PEG per mol protein
matrix attachment
secondary amine linkage.
storage temp.
−20°C
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Application
Pulmonary artery endothelial cells treated with PEG-catalase showed effective inhibition of 20- HETE (hydroxyeicosatetraenoic acid)-induced increase in fluorescence. However, the experiment excludes potential nonspecific fluorescence of DCF (dichlorofluorescein). This experiment studied the effect of 20-HETE on superoxide production and NADPH oxidase activation.[1]
Biochem/physiol Actions
Catalase from bovine liver catalyzes the decomposition of H2O2 into water and oxygen. It is a tetramer consisting of four equal subunits with a molecular weight of 60 kDa each. Each subunit contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Catalase activity is constant over the pH range of 4.0-8.5. The pI is found to be 5.4. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule. It does not require any activators.
Packaging
Package size based on protein content
Other Notes
Catalase from bovine liver coupled to methoxy-polyethylene glycol.
Physical form
Contains PEG plus 5% citrate buffer salts
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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I would like to know the function of PEG with the catalase for this product.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the specific activity of Catalase-polyethylene glycol, Product C4963?
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The certificate of analysis on our website will have lot-specific activity information.
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What can I use to solubilize Catalase-polyethylene glycol, Product C4963?
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It is soluble in water at a concentration of 1 mg/mL.
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What is the solution stability of Catalase-polyethylene glycol, Product C4963?
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We generally recommend preparing fresh solutions of Catalase-PEG. If stock solutions must be stored, we recommend first dissolving in water, then diluteing with an equal volume of glycerol. This 50% glycerol solution may be kept in the freezer at -20°C. Aqueous solutions held at 37°C were found to lose approximately half of the catalase activity after about 10 days.
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