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A7653

L-Alanine Dehydrogenase from Bacillus subtilis

buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)

Synonym(s):

L-Alanine: NAD+ oxidoreductase (deaminating)

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100 units
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MXP 7,009.00

About This Item

CAS Number:
9029-06-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-847-9
MDL number:
MFCD00131441
EC Number:
1.4.1.1 (BRENDA, IUBMB)
Specific activity:
~30 units/mg protein (Lowry)
Biological source:
Bacillus subtilis

MXP 7,009.00

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biological source

Bacillus subtilis

Quality Segment

200

form

buffered aqueous glycerol solution

specific activity

~30 units/mg protein (Lowry)

foreign activity

LDH ~1% (using pyruvate as substrate)

storage temp.

−20°C

Application

L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection [1].

Biochem/physiol Actions

L-Alanine dehydrogenase is an A-stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate, and NADH are released in that specific order.[1] Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.[2]

Physical form

Solution in 50% glycerol containing 10 mM potassium phosphate buffer, pH 7.7

Other Notes

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH3 per min at pH 10.0 at 25 °C.

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L-Alanine Dehydrogenase from Bacillus subtilis buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)

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Sigma-Aldrich

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L-Leucine Dehydrogenase from Bacillus cereus

L-Amino Acid Oxidase from Crotalus adamanteus Type IV, ≥4.0 units/mg protein, aqueous suspension

Sigma-Aldrich

A9378

L-Amino Acid Oxidase from Crotalus adamanteus

biological source

Bacillus subtilis

biological source

Bacillus subtilis

biological source

-

biological source

-

specific activity

~30 units/mg protein (Lowry)

specific activity

≥20 units/mg protein (Lowry)

specific activity

≥60 units/mg protein

specific activity

≥4.0 units/mg protein

form

buffered aqueous glycerol solution

form

ammonium sulfate suspension

form

lyophilized powder

form

aqueous suspension

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

foreign activity

LDH ~1% (using pyruvate as substrate)

foreign activity

-

foreign activity

-

foreign activity

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000380060
0000279721
0000380060
0000279721
11181151

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Global Trade Item Number

SKUGTIN
A7653-100UN04061833386026

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