21878

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester

BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)

• Synonym(s): 5(6)-FAM SE, FLUOS
• Empirical Formula (Hill Notation): C₂₅H₁₅NO₉
• CAS Number: 117548-22-8
• Molecular Weight: 473.39
• MDL number: MFCD04119713
• UNSPSC Code: 12352108
• PubChem Substance ID: 57647947
• NACRES: NA.32

Properties

• product line: BioReagent
• Assay: ≥80% (HPLC)
• form: powder
• solubility: DMF: soluble; DMSO: soluble
• fluorescence: λ_ex 492 nm; λ_em 517 nm in DMF
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: OC(=O)c1ccc(cc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O.OC(=O)c6cccc(C(=O)ON7C(=O)CCC7=O)c6C8=C9C=CC(=O)C=C9Oc%10cc(O)ccc8%10
• InChI: 1S/2C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)18-9-12(1-4-15(18)24(31)32)25(33)35-26-21(29)7-8-22(26)30;27-12-4-6-14-18(10-12)34-19-11-13(28)5-7-15(19)22(14)23-16(24(31)32)2-1-3-17(23)25(33)35-26-20(29)8-9-21(26)30/h1-6,9-11,27H,7-8H2,(H,31,32);1-7,10-11,27H,8-9H2,(H,31,32)
• InChI key: YVTPVJDECOUTJW-UHFFFAOYSA-N

Description

Application

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5(6)-FAM SE, FLUOS) may be used to prepare fluorescent derivatives of proteins such as tubulin and for the preparation of a phenytoin tracer for the determination of this and other drugs in serum and plasma by fluorescence polarization immunoassay (FPIA) and laser-induced fluorescence detection.

Fluorescent label for primary and secondary amine groups on proteins and other biomolecules.

Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA)

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves