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Merck

17490

Sigma-Aldrich

Bromocresol Purple

for microscopy (Hist., Vit.), indicator (pH 5.2-6.8)

Synonym(s):

5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form

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About This Item

Empirical Formula (Hill Notation):
C21H16Br2O5S
CAS Number:
Molecular Weight:
540.22
Beilstein:
359618
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

grade

for microscopy (Hist., Vit.)
indicator (pH 5.2-6.8)

form

powder

technique(s)

titration: suitable

pH

5.2-6.8, yellow to purple

mp

240 °C (dec.) (lit.)

solubility

hot ethanol: 0.1 g/10 mL

εmax

≥400 at 414-424 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4

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General description

Bromocresol purple is a pH indicator, which changes color from yellow (at low pH 5.2) to violet (above pH 6.8).[1] It aids in determining the dead cell count for cells with plasma membrane damage. Bromocresol purple is widely used as a fluorescent stain in yeast cells. Cells with an intact plasma membrane are not stained, while damaged cells are seen as blue-grey ghosts.[2]

Application

Spectrophotometric determination of serum-albumin

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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