137260

BIS-TRIS

EMPROVE^® EVOLVE

• Synonym(s): BIS TRIS, 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
• Empirical Formula (Hill Notation): C₈H₁₉NO₅
• CAS Number: 6976-37-0
• Molecular Weight: 209.24
• Beilstein: 2205275
• MDL number: MFCD00002853
• UNSPSC Code: 12161705

Properties

• biological source: synthetic
• Quality Level: 400
• product line: EMPROVE^® EVOLVE
• form: powder
• technique(s): cell culture | mammalian: suitable
• impurities: Endotoxin, microbial, and trace metals, tested
• pH: 5.8-7.2
• useful pH range: 5.8-7.2
• pKa (25 °C): 6.5
• pKa: 6.5
• solubility: water: 0.1 M, clear, colorless (aqueous)
• suitability: suitable for manufacturing use
• foreign activity: Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
• storage temp.: 2-30°C
• SMILES string: OCCN(CCO)C(CO)(CO)CO
• InChI: 1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
• InChI key: OWMVSZAMULFTJU-UHFFFAOYSA-N

Description

Application

Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological based formulations.Bis Tris is used in a wide variety of biological applications including culture media, electrophoresis separations and diagnostic reagent formulations.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable