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H3136

Sigma-Aldrich

Hematoxylin

certified by the Biological Stain Commission

Synonym(s):

Natural Black 1

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25 G
MXP 5,018.00
100 G
MXP 16,046.00

MXP 5,018.00


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25 G
MXP 5,018.00
100 G
MXP 16,046.00

About This Item

Empirical Formula (Hill Notation):
C16H14O6 · xH2O
CAS Number:
Molecular Weight:
302.28 (anhydrous basis)
Colour Index Number:
75290
Beilstein:
91400
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

MXP 5,018.00


In StockDetails


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grade

certified by the Biological Stain Commission

Quality Level

form

powder

mp

200 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1cc2C[C@@]3(O)COc4c(O)c(O)ccc4[C@H]3c2cc1O

InChI

1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1

InChI key

WZUVPPKBWHMQCE-XJKSGUPXSA-N

Gene Information

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General description

Hematoxylin is a basophilic dye extracted from the tree Heamatoxylum campechianum. It specifically binds to acidic compounds and thus is useful for staining cell nuclei which contain acidic substances: DNA and RNA. Hematoxylin, when oxidized forms hematein, which has thick pale blue purple colour. Hematoxylin is often used along with eosin, in histological study under light microscope.[1]

Application

Hematoxylin has been used:
  • to visualize anatomical features and to detect nucleus locus coeruleus adjacent to the periaqueductal gray region in brainstem samples[2]
  • as a nuclear counterstain[3] to visualize the nucleus, adipocytes were counterstained with a hematoxylin reagent[4]
  • to stain lung tissue for histological analysis[5]

Suitability

Certified for use in Weigert′s iron hematoxylin, Mayer′s hemalum, Harris hemalum and in Heidenhain′s technique

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Histologic validation of locus coeruleus MRI contrast in post-mortem tissue.
Keren NI
Neuroimage, 113, 235-235 (2015)
Expression of matrilin-2 in liver cirrhosis and hepatocellular carcinoma
Szabo E, et al.
Pathology Oncology Research, 14(1), 15-22 (2008)
Expression of matrilin-2 in liver cirrhosis and hepatocellular carcinoma.
Szabo E
Pathology Oncology Research, 14, 15-15 (2008)
Techniques in Aquatic Toxicology (2005)
S-nitrosylation of fatty acid synthase regulates its activity through dimerization.
Choi MS
Journal of Lipid Research, 57, 607-607 (2016)

Questions

1–2 of 2 Questions  
  1. what is difference between H9627 and this product?

    1 answer
    1. These products are physically the same compound. However, this product is certified for use in Wiegert's iron hematoxylin, Mayer's hemalum, Harris hemalum, and in Heidenhain's technique by the Biological Stain Commission. Whereas the H9627 has not been certified or tested for any specific staining procedure.

      Helpful?

  2. Is the certified hematoxylin dye, H3136, intended for preparing a specific formulation of hematoxylin solution?

    1 answer
    1. Product H3136, which is certified by the Biological Stain Commission, is a hematoxylin powder intended for customers to formulate the hematoxylin solution of their choice. There are numerous hematoxylin formulations available in the literature, with some being more commonly used than others. The expectation is that a certified hematoxylin should be suitable for preparing any type of hematoxylin solution.

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