D9805

Fast Blue B Salt

Dye content ~95 %, Powder

• Synonym(s): o-Dianisidine bis(diazotized) zinc double salt, Azoic Diazo No. 48, DBB, Diazo Blue B, Naphthanil Diazo Blue B
• Linear Formula: C₁₄H₁₂N₄O₂Cl₂ · ZnCl₂
• CAS Number: 14263-94-6
• Molecular Weight: 475.47
• Colour Index Number: 37235
• Beilstein: 1499067
• EC Number: 238-153-2
• MDL number: MFCD00075203
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894113
• NACRES: NA.47

Properties

• Product Name: Fast Blue B Salt, Dye content ~95 %
• description: Technical grade
• Quality Level: 200
• form: powder
• composition: Dye content, ~95%
• technique(s): microbe id | staining: suitable
• color: yellow to dark brown
• mp: >300 °C (lit.)
• solubility: H₂O: 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: [Cl-].[Cl-].Cl[Zn]Cl.COc1cc(ccc1[N+]#N)-c2ccc([N+]#N)c(OC)c2
• InChI: 1S/C14H12N4O2.4ClH.Zn/c1-19-13-7-9(3-5-11(13)17-15)10-4-6-12(18-16)14(8-10)20-2;;;;;/h3-8H,1-2H3;4*1H;/q+2;;;;;+2/p-4
• InChI key: GPPKNJIWDULNQH-UHFFFAOYSA-J

Description

Application

• Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.[1]
• It has been used as a spraying agent in thin layer chromatography for the identification of separated components.[2]
• It has also been used for the determination of the lipase activity.[3][4]

Biochem/physiol Actions

Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye.[5] Similarly, fast blue B is also used for α- and β-glucosidase activity.[6]

Safety Information

• Pictograms: GHS02,GHS08
• Signal Word: Danger
• Hazard Statements: H242,H350
• Precautionary Statements: P202 - P210 - P235 - P308 + P313 - P370 + P378 - P403
• Hazard Classifications: Carc. 1B - Self-react. C
• Storage Class Code: 5.2 - Organic peroxides and self-reacting hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves