glucose, 20 mM magnesium chloride, 10 mM magnesium sulfate, 10 mM potassium chloride, 2.5 mM sodium chloride, 10 mM tryptone, 20 g/L yeast extract, 5 g/L
technique(s)
microbiological culture: suitable
final pH
7.0±0.2 (25 °C)
application(s)
food and beverages life science and biopharma microbiology
storage temp.
2-8°C
suitability
nonselective for Escherichia coli nonselective for coliforms
Application
SOC Broth is a sterile solution for the recovery of electroporated Escherichia coli cells.[1]
Preparation Note
Immediately after electroporation add 1 ml sterile SOC Medium to the transformed cells and incubate for 30-60 minutes at 37°C with a moderate shaking .
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Journal of molecular biology, 166(4), 557-580 (1983-06-05)
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth
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