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Roche

Formate Dehydrogenase

from Candida boidinii

Synonym(s):

FDH, Formate:NAD oxidoreductase

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About This Item

Enzyme Commission number:
1.2.1.2 (BRENDA, IUBMB)
UNSPSC Code:
12352204

biological source

yeast (Candida boidini)

Quality Level

100

recombinant

expressed in E. coli

form

lyophilized

specific activity

~0.4 units/mg protein (At 25 °C with formate as the substrate.)

mol wt

74 kDa

packaging

pkg of 250 U (10837016001)
pkg of 80 U (10244678001)

manufacturer/tradename

Roche

concentration

≥10-20 % (w/w)

technique(s)

activity assay: suitable

color

off-white

optimum pH

7.5-8.5

solubility

water: soluble 10 g/L

suitability

suitable for gel electrophoresis

UniProt accession no.

O13437

application(s)

life science and biopharma

foreign activity

ADH <0.0500%
LDH <0.0500%
MDH <0.1000%

storage temp.

2-8°C

Related Categories

General description

Formate:NAD+ oxidoreductase
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.

Specificity

Substrate Specificity and Km:
Formate dehydrogenase reacts only with formate (Km = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (Km 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.

Application

Most widely used in cofactor recycling systems for NADH.

Quality

Contaminants: <0.005% LDH and ADH each, <0.1% MDH

Unit Definition

One unit (U) formate dehydrogenase will oxidize 1 μmol of formic acid to CO2 in 1 minute at +25 °C and pH 7.6. The above assay produces 1 mmol of NADH per mmol of formate oxidized.

Storage and Stability

Store dry

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Knut Erik Hovda et al.
Scandinavian journal of clinical and laboratory investigation, 75(7), 610-614 (2015-07-24)
The standard diagnostic approach to methanol poisoning is chromatographic measurement of methanol on centrally placed stationary equipment. Methanol poisoning in places where such equipment is unavailable is thus often not diagnosed. Methanol is metabolized to a toxic metabolite, formate; the
Katja Schirwitz et al.
Protein science : a publication of the Protein Society, 16(6), 1146-1156 (2007-05-26)
The understanding of the mechanism of enzymatic recovery of NADH is of biological and of considerable biotechnological interest, since the essential, but expensive, cofactor NADH is exhausted in asymmetric hydrogenation processes, but can be recovered by NAD(+)-dependent formate dehydrogenase (FDH).

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