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11243217001

Roche

Laminin

from mouse Engelbreth-Holm-Swarm (EHS) sarcoma

Synonym(s):

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

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About This Item

CAS Number:
114956-81-9
MDL number:
MFCD00081739
UNSPSC Code:
12352207

biological source

mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)

Quality Level

100

sterility

sterile; sterile-filtered

Assay

≥90% (SDS-PAGE)

form

solution

mol wt

900 kDa

packaging

pkg of 1 mg (2 ml)

manufacturer/tradename

Roche

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

Q61292

storage temp.

−20°C

Gene Information

mouse ... Lamb2(16779)

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General description

Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from α, β and γ chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.

Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.

Application

Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Quality

Purity: ≥90% (SDS-PAGE) (as laminin nidogen-complex, 1:1)

Sequence

Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated.

Physical form

Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile
Solution, filtered through 0.2 ?m pore size membrane

Preparation Note

Working concentration: 2 to 5 μg/cm2
2 to 5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.

Other Notes

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

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