Phenol red is unlikely to interfere with the detection of this dye. However, it's unnecessary to have phenol red in the cell culture medium at this point. Highly recommend using cell culture medium without phenol red.
SCT119
BioTracker 650 Red Nuclear Dye
Live cell imaging red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
Synonym(s):
Live cell imaging probe, Live cell nuclear probe
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.47
Pricing and availability is not currently available.
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technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
General description
The nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cell′s genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.
The BioTracker 650 Red Nuclear Dye is a cell-membrane permeable red fluorescent DNA dye that specifically stains nuclei in live or fixed cells. It has excellent specificity for DNA without the need for a wash step, and it has low toxicity for live cell imaging. The dye is supplied with a vial of verapamil, an efflux pump inhibitor that may improve probe retention and live cell staining in certain cell types.
Spectral Properties
Absorbance: 650nm
Emission: 675nm
The BioTracker 650 Red Nuclear Dye is a cell-membrane permeable red fluorescent DNA dye that specifically stains nuclei in live or fixed cells. It has excellent specificity for DNA without the need for a wash step, and it has low toxicity for live cell imaging. The dye is supplied with a vial of verapamil, an efflux pump inhibitor that may improve probe retention and live cell staining in certain cell types.
Spectral Properties
Absorbance: 650nm
Emission: 675nm
Application
Live cell fluorescent imaging
Live cell imaging red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
Components
1) 1 vial of 50µL BioTracker 650 Red Nuclear Dye (1000X in DMSO) (CS224577)
2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)
2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)
Quality
Spectral Properties
Absorbance: 650nm
Emission: 675nm
Absorbance: 650nm
Emission: 675nm
Physical form
Liquid
Storage and Stability
Store BioTracker 650 Red Nuclear Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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Is there a risk of interference from phenol red in cell culture medium with the detection of this dye, as the protocol recommends dilution in cell culture medium?
1 answer-
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Is this dye compatible to image live migrating HL60 cells?
1 answer-
This product is suitable for live cell imaging. The HL60 cell line has not been specifically tested, however there is no reason to expect it will not work. While this stain is markedly more photostable that DAPI and other similar dyes, the retention time has not been determined. The addition of verapamil is recommended to improve retention time. See the link below for the product data sheet.
The protocol can be found on page 2:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/211/919/sct119ds.pdfHelpful?
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