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PC474

Sigma-Aldrich

Anti-PCNA (247-261) (Ab-5) Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-Proliferating Cell Nuclear Antigen

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PCNA(5111)

General description

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~36-37 kDa PCNA protein.
Recognizes the ~37 kDa PCNA protein in MCF-7 cells.
This Anti-PCNA (247-261) (Ab-5) Rabbit pAb is validated for use in Immunoprecipitation for the detection of PCNA (247-261) (Ab-5).

Immunogen

Human
a synthetic peptide corresponding to amino acids 247-261 of human PCNA

Application


Immunoprecipitation (1:250-1:1000)

Warning

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
MCF7 cells

Other Notes

Miyachi, K., et al. 1990. J. Immunol.121, 2228.
Waseem, N.H. and Lane, D.P. 1990. Science96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86, 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Will immunoprecipitate PCNA when complexed with other proteins. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

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Richard Belvindrah et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(9), 3594-3607 (2002-04-30)
mCD24, a glycosylphosphatidylinositol-anchored highly glycosylated molecule, is expressed on differentiating neurons during development. In the adult CNS, its expression is restricted to immature neurons located in two regions showing ongoing neurogenesis: the subventricular zone (SVZ) of the lateral ventricle pathway

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