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Sigma-Aldrich

Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, sheep, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Htr2A(29595)

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa serotonin 2A (5-HT2A) receptor.
Recognizes the ~53 kDa serotonin 2A (5-HT2A) receptor.
This Anti-Serotonin 2A Receptor Rabbit pAb is validated for use in Frozen Sections, WB, Free Floating Sections, Paraffin Sections for the detection of Serotonin 2A Receptor.

Immunogen

Rat
a synthetic peptide corresponding to amino acids 22-41 of rat 5-HT2A receptor

Application


Frozen Sections (1:300-1:500)
Immunoblotting (1:500-1:1000)
Free Floating Sections (see application references)
Paraffin Sections (see application references; heat pre-treatment required)

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 20 mM sodium phosphate, 1% BSA, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Antibody specificity was examined in the rat amygdala, cortex, and hippocampus. Detection of primary antibody with ABC according to manufacturers directions. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. Addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solutions allows further dilution of the primary antibody to approximately 1:1000 or greater. For staining paraffin sections, heating in 10 mM citrate buffer is required (see application references). Antibody should be titrated for optimal results in individual systems.
Leonard, B.E. 1996. Psychother. Psychosom.65, 66.
Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol.8, 175.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Gongliang Zhang et al.
Neuropharmacology, 109, 332-340 (2016-04-27)
The rodent hippocampus supports non-spatial object memory. Serotonin 5-HT2A receptors (5-HT2AR) are widely expressed throughout the hippocampus. We previously demonstrated that the activation of 5-HT2ARs enhanced the strength of object memory assessed 24 h after a limited (i.e., weak memory) training
Lizhu Jiang et al.
Frontiers in molecular neuroscience, 12, 317-317 (2020-02-11)
Spaced training is robustly superior to massed training, which is a well-documented phenomenon in humans and animals. However, the mechanisms underlying the spacing effect still remain unclear. We have reported previously that spacing training exerts memory-enhancing effects by inhibiting forgetting
Brian R Noga et al.
Journal of neurophysiology, 102(3), 1560-1576 (2009-07-03)
Monoamines are strong modulators and/or activators of spinal locomotor networks. Thus monoaminergic fibers likely contact neurons involved in generating locomotion. The aim of the present study was to investigate the serotonergic innervation of locomotor-activated neurons within the thoraco-lumbar spinal cord
Min Su et al.
The European journal of neuroscience, 44(4), 2129-2138 (2016-06-12)
Clinical and preclinical studies have implicated serotonin (5-HT) and the 5-HT2A receptor (5-HT2AR) in the pathogenesis of medication-overuse headache (MOH). However, with no appropriate animal model to study this phenomenon it is difficult to differentiate the effects of chronic exposure
Youngjae Ryu et al.
Scientific reports, 11(1), 7120-7120 (2021-03-31)
In the majority of spinal cord injury (SCI) patients, spasticity develops in the subacute phase and chronically persists with muscle hypertonia. Among various pathological conditions underlying spasticity, upregulated expression of 5-HT receptors (5-HTR) on the spinal motor neurons due to

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