MABT1366

Anti-CRB3A Antibody, clone 1E6

clone 1E6, from rat

• Synonym(s): Protein crumbs homolog 3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1E6, monoclonal
• species reactivity: human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology), Drosophila (based on 100% sequence homology)
• packaging: antibody small pack of 25 μg
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_631900
• UniProt accession no.: Q9BUF7
• target post-translational modification: unmodified
• Gene Information: human ... CRB3(92359)

Description

General description

Protein crumbs homolog 3 (UniProt: Q9BUF7) is encoded by the CRB3 (also known as UNQ588/PRO1158) gene (Gene ID: 92359) in human. Mammalian cells contain three Crb orthologs. CRB1 is primarily expressed in the brain, cornea, and retina. CRB2 is expressed in lung, kidney, retina, colon and mammary glands. It is also expressed in at moderate levels in Expressed at moderate levels in liver, spleen, pancreas, placenta, and prostate tissue. CRB3 is present in epithelial tissues and is present in two isoforms, CRB3A and CRB3B. CRB3 is a single-pass type I membrane protein that is reported to control epithelial cell polarization. CRB3A is apically localized and promotes apical membrane growth and is and is essential for the formation of tight junctions in epithelial cells. It also prevents the epithelial-to-mesenchymal transition (EMT), which is known to contribute to tumor progression. CRB3 is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off. The mature form contains an extracellular domain (aa 27-59), a transmembrane domain (aa 60-80), and a cytoplasmic tail (aa 81-120), which contains a PDZ-domain binding motif (aa 117-120) that interacts with the Maguk protein, PALS1, which recruits PALS1-associated tight junction (PATJ) protein into the CRB3A complex. Both PALS1 and PATJ play a critical role in epithelial cell polarity and tight junction formation. CRB3A is shown to control and properly localize RhoA signaling that is required for CRB3A-dependent reorganization of the actin cytoskeleton. (Ref.: Loie, E., et al (2015). Mol. Cell Biol. 35(19); 3423-3435).

Specificity

Clone 1E6 is a rat monoclonal antibody that detects protein crumbs homolog 3 in human cells. it targets an epitope with in 15 amino acids from the C-terminal region.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Protein crumbs homolog 3 (CRB3).

Application

Anti-CRB3A, clone 1E6, Cat. No. MABT1366, is a rat monoclonal antibody that detects Protein crumbs homolog 3 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Cell Structure

Western Blotting Analysis: 2 µg/mL from a representative lot detected CRB3A in Caco2 cell lysates over-expressing Crumbs 3A (positive) versus lysates from Caco2 cells silenced with siRNA (negative).

Western Blotting Analysis: A representative lot detected CRB3A in Western Blotting applications (Loie, E., et. al. (2015). Mol Cell Biol. 35(19):3423-35).

Quality

Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected CRB3A in human kidney tissue sections.

Target description

~35 kDa observed; 12.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.