MABS2279

Anti-Serpin B13 Antibody, clone B34

• Synonym(s): Serpin family B member 13
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: B34, monoclonal
• mol wt: calculated mol wt 44.32 kDa
• purified by: using protein G
• species reactivity: mouse
• packaging: antibody small pack of 100 μg
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• isotype: IgG2aκ
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_766440
• UniProt accession no.: Q8CDC0
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: mouse ... Serpinb13(241196)

Description

General description

Serpin B13 (UniProt: Q8CDC0; also known as Serpin family B member 13 ) is encoded by the Serpinb13 gene (Gene ID: 241196) in murine species. Serpin B13 is a cytoplasmic protein that plays a role in the proliferation or differentiation of keratinocytes. Two isoforms of Serpin B13 have been described that are generated by alternative splicing. Serpin B13 acts as a serine proteinase inhibitor and studies have shown a strong induction of serpin B13 in the pancreas of mice during the first few weeks of life. Cathepsin L and K appear to be the targets of serpin B13 action. Serpin B13 is expressed in pancreas with low levels of expression in pancreatic islets and larger expression in the exocrine pancreas. Secretion of anti-serpin B13 autoantibodies in young diabetes-prone non-obese diabetic (NOD) mice is shown to be associated with reduced inflammation in pancreatic islets and a slower progression to autoimmune diabetes. Anti-serpin B13 autoantibodies are shown to protect NOD mice against diabetes before 16 weeks of age. Reduced expression of serpin B13 autoantibodies is associated with development of type I diabetes in human before 5 years of age. Mice injected with monoclonal anti-serpin B13 display few inflammatory cells in the islets and rapid recovery from recent onset diabetes. (Ref.: Kryvalap, Y., et al. (2021). Sci. Transl. Med. 13(588); eabf1587; Czyzyk, J. et al. (2012). J. Immunol. 188(12); 6319-6327).

Specificity

Clone B34 is a mouse monoclonal antibody that detects mouse Serpin B13.

Immunogen

Recombinant full-length mouse Serpin B13.

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in mouse skin tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Serpin B13 in mouse skin tissue sections.

Tested Applications

Immunohistochemistry Applications: A representative lot detected Serpin B13 in Immunohistochemistry applications (Kryvalap, Y., et al. (2021). Sci Transl Med.13(588):eabf1587).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Serpin B13, clone B34, Cat. No. MABS2279, is a mouse monoclonal antibody that detects Serpin B13 and is tested for use in Immunohistochemistry (Paraffin).

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable