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MABF152B

Sigma-Aldrich

Anti-F4/80 (mouse), APC, clone BM8.1 Antibody

clone BM8.1, from rat, Allophycocyanin conjugate

Synonym(s):

EGF-like module-containing mucin-like hormone receptor-like 1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EMR1 hormone receptor, F4/80

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

conjugate

Allophycocyanin conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

BM8.1, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable

isotype

IgG2aκ

UniProt accession no.

Q61549

target post-translational modification

unmodified

Gene Information

mouse ... Adgre1(13733)

General description

The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This rat monoclonal Anti-F4/80 Antibody (mouse), APC, clone BM8.1, Cat. No. MABF1523 is validated for use in Flow Cytometry, for the detection of F4/80.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg from a representative lot detected F4/80 in one million C57Bl/6 splenocytes stained with FITC Anti-mouse CD11b.

Physical form

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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