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Merck
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71009-M

Millipore

CytoBuster Protein Extraction Reagent

Simple extraction of soluble protein from mammalian and insect cells

Synonym(s):

Cell Lysis Reagent, Protein Extraction Buffer

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About This Item

UNSPSC Code:
41106510
NACRES:
NA.77

Quality Level

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

storage temp.

10-30°C

General description





The CytoBuster Protein Extraction Reagent is a proprietary formulation of detergents optimized for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of CytoBuster Protein Extraction Reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw. CytoBuster Protein Extraction Reagent has been specifically formulated for utilization in Western blotting, immunoprecipitation, and kinase/phosphatase assays. The reagent is compatible with protease inhibitors, kinase inhibitors, and phosphatase inhibitors.

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Articles

CytoBuster Protein Extraction Reagent is a formulation of detergents prepared for the efficient extraction of soluble proteins from mammalian cells

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An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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