574795
TBE Buffer, 10X, Molecular Biology Grade
A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.
Synonym(s):
Tris-Borate-EDTA Buffer
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About This Item
UNSPSC Code:
41105319
NACRES:
NA.25
Recommended Products
grade
Molecular Biology
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
foreign activity
DNase, RNase, protease, none detected
shipped in
ambient
storage temp.
15-25°C
General description
A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.
Tris, borate, and ethylenediaminetetraacetic acid (TBE) buffer is used to perform agarose gel electrophoresis of DNA and RNA. It consists of a weak acid that exists in neutral and anionic forms (e.g., COOH and COO- species). Tris is a weak base, which occurs in either neutral or cationic forms (Tris-NH2 and Tris-NH3+). These ions maintain a low conductivity medium, transmit the electrical current, and buffer the pH. Ethylenediaminetetraacetic (EDTA) although not essential is added as a preventative since it chelates Mg2+ ions and inactivates potential DNA nucleases. TBE allows an effective resolution of DNA fragments and slightly improves the separation of smaller fragments. It also stabilizes nucleic acids against enzymatic degradation.
Application
TBE Buffer, 10X, Molecular Biology Grade-Calbiochem has been used as a component of polyacrylamide sequencing gels for DNA electrophoresis using amplified fragment length polymorphism (AFLP).
Warning
Toxicity: Irritant (B)
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 1B
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric
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