Recommended Products
Assay
≥90% (SDS-PAGE)
Quality Level
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<1.0 EU/μg protein Endotoxin
shipped in
ambient
storage temp.
−20°C
General description
Recombinant, mouse keratinocyte growth factor (KGF) consisting of amino acids 32-194 and expressed in E. coli. KGF plays an essential role in development, tumorigenesis, angiogenesis, morphogenesis, and wound healing. Mature mouse KGF amino acid sequence is 96% identical with rat, bovine, ovine, equine, and human forms and is active across species.
Biochem/physiol Actions
ED₅₀ = 10-50 ng/ml as measured in a cell proliferation assay using 4MBr-5 monkey epithelial cells.
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 0.2 µm sterile-filtered MOPS, Na₂SO₄, EDTA, DTT BSA.
Reconstitution
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 250 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Other Notes
Belleudi, F., et al. 2007. Traffic8, 1854.
Finch, P.W. and J.S. Rubin. 2006. J. Natl. Cancer Inst.98, 812.
de Georgi, V., et al. 2007. Dermatol. Clin.25, 477.
Niu, J., et al. 2007. J. Biol. Chem.282, 6001.
Werner, S., et al. 2007. J. Invest. Dermatol.127, 998.
Cardinali, G., et al. 2005. J. Invest. Dermatol.125, 1190.
Geer, D.J., et al. 2005. Am. J. Pathol.167, 1575.
Hsu, YR., et al. 1999. Biochemistry38, 2523.
Qiao, J., et al. 1999. Development126, 547.
Werner, S., 1998. Cytokine Growth Factor Rev.9, 153.
Guo, L., et al. 1996. Genes Dev.10, 165.
Mason, I.J., et al. 1994. Mech. Dev.45, 15.
Finch, P.W. and J.S. Rubin. 2006. J. Natl. Cancer Inst.98, 812.
de Georgi, V., et al. 2007. Dermatol. Clin.25, 477.
Niu, J., et al. 2007. J. Biol. Chem.282, 6001.
Werner, S., et al. 2007. J. Invest. Dermatol.127, 998.
Cardinali, G., et al. 2005. J. Invest. Dermatol.125, 1190.
Geer, D.J., et al. 2005. Am. J. Pathol.167, 1575.
Hsu, YR., et al. 1999. Biochemistry38, 2523.
Qiao, J., et al. 1999. Development126, 547.
Werner, S., 1998. Cytokine Growth Factor Rev.9, 153.
Guo, L., et al. 1996. Genes Dev.10, 165.
Mason, I.J., et al. 1994. Mech. Dev.45, 15.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service