126741

Alcalase^® Enzyme, Bacillus licheniformis

• Synonym(s): Protease from Bacillus licheniformis, subtilisin A
• Enzyme Commission number: 3.4.21.14 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.56

Properties

• biological source: Bacillus licheniformis
• Quality Level: 100
• form: liquid
• specific activity: ≥0.75 Anson units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• technique(s): activity assay: suitable
• color: dark brown
• application(s): life science and biopharma
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Alcalase^® is a serine endopeptidase that consists primarily of subtilisin A.

Application

Alcalase^® Enzyme, Bacillus licheniformis has been used in the proteolytic digestion and extraction of polysaccharides in various sponge species. It has also been used as an endoprotease enzyme in enzymatic hydrolysis for the production of fish protein hydrolysate.

Biochem/physiol Actions

Alcalase^® is suitable for the hydrolysis of proteins with an optimal pH of 7.0-8.5. It is used as an additive in detergent, dehairing, and bating leather. Alcalase is also used in meat tenderizing, cheese flavor development, baked manufacture, and improving the digestibility of animal feeds. Commercial Alcalase^® comprises a liquid enzymatic preparation containing 50% glycerol, 41% water, and 9% protease extract from Bacillus licheniformis.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will release 1.0 µmol L-tyrosine from hemoglobin per min at 25°C, pH 7.5.

Reconstitution

Please refer to the vial label for lot-specific density.

Legal Information

Alcalase is a registered trademark of Novozymes Corp.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08,GHS05
• Signal Word: Danger
• Hazard Statements: H318,H334,H412
• Precautionary Statements: P261 - P273 - P280 - P284 - P305 + P351 + P338 - P501
• Hazard Classifications: Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable