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1.15389

Supelco

TLC plates, Silica gel 60 RP-18 F₂₅₄s

pkg of 25 plates, plate L × W 20 cm × 20 cm, glass support

Synonym(s):

Silica Gel 60 RP-18 F₂₅₄s TLC Plates

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About This Item

UNSPSC Code:
41115711
NACRES:
NB.21

material

glass support
silica gel 60 matrix

Quality Level

100

feature

binder Organic Polymer
fluorescent indicator

packaging

pkg of 25 plates

technique(s)

thin layer chromatography (TLC): suitable

layer thickness

250 μm

plate L × W

20 cm × 20 cm

particle size

10-12 μm

pore size

60 Å medium pore diameter

storage temp.

2-30°C

General description

25 Glass plates 20 x 20 cm
RP modified plates serve two purposes: act as a pilot method for HPLC and allow to choose various solvent system for special separations. Our RP-modified silica layers are well suited for many separation challenges that unmodified silica cannot overcome. These layers use aqueous solvent systems to separate extremely non-polar substances and analyze particular polar substances that can adapt to ion-pair chromatography. What’s more, RP-modified silica layers are less dependent on atmospheric humidity. Unlike unmodified silica, RP-phases do not exhibit catalytic activity. This makes them the plates of choice for unstable substance that tend to experience oxidative degradation.

Application

Glass TLC plates, RP-18 modified silica gel coated with flourescent indicator F254s. The RP-18 modified silica gel TLC plates come in a quantity of 25 size 20x20 cm for thin layer chromatography applications.

Linkage

Replaces: 15389-7; 15389

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 9.7 - 11.7 µm
Layer thickness: 200 - 270 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Cholesterin test
hRf-values
- cholesterin, cholesterin test:
30 - 38
B) Steroid test
- methyltestosterone, steroid test: 21 - 31
- Reichstein S, steroid test: 50 - 60
- hydrocortisone, steroid test: 70 - 80
Typical value determined on an activated plate (120°C, 15 min)
Eluent a) aceton/ water (95/5 v/v)
Eluent b) acetonitrile/ water (70/30 v/v)

Storage Class Code

13 - Non Combustible Solids

Certificates of Analysis (COA)

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High Performance Thin Layer Chromatography Plates

HPTLC plates provide superior separation, speed, and sensitivity, making them ideal for advanced qualitative and quantitative analysis.

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