1.13724
HPTLC plates, Silica gel 60 RP-18 F₂₅₄s
pkg of 25 plates, plate L × W 10 cm × 10 cm, glass support
Synonym(s):
Reversed Phase High Performance TLC Plates
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About This Item
UNSPSC Code:
41115711
NACRES:
NB.21
Pricing and availability is not currently available.
Recommended Products
material
glass support
silica gel 60 matrix
Quality Level
feature
binder Organic Polymer
fluorescent indicator
packaging
pkg of 25 plates
technique(s)
thin layer chromatography (TLC): suitable
layer thickness
200 μm
plate L × W
10 cm × 10 cm
particle size
5-6 μm
pore size
60 Å medium pore diameter
storage temp.
2-30°C
General description
25 Glass plates 10 x 10 cm
RP modified plates serve two purposes: act as a pilot method for HPLC and allow to choose various solvent system for special separations. Our RP-modified silica layers are well suited for many separation challenges that unmodified silica cannot overcome. These layers use aqueous solvent systems to separate extremely non-polar substances and analyze particular polar substances that can adapt to ion-pair chromatography. What’s more, RP-modified silica layers are less dependent on atmospheric humidity. Unlike unmodified silica, RP-phases do not exhibit catalytic activity. This makes them the plates of choice for unstable substance that tend to experience oxidative degradation.
Application
Glass HPTLC Silica gel 60 RP-18 F₂₅₄s plates. Silica gel HPTLC plates size 10 x 10 cm, 25 sheets.
Linkage
Replaces: 13724-5; 13724
Analysis Note
Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Cholesterin test
hRf-values
- cholesterin, cholesterin test:
11 - 23
B) Steroid test
- methyltestosterone, steroid test: 20 - 40
- Reichstein S, steroid test: 50 - 70
- hydrocortisone, steroid test: 65 - 85
Typical value determined on an activated plate (120°C, 15 min)
Eluent a) aceton/ water (95/5 v/v)
Eluent b) acetonitrile/ water (70/30 v/v)
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Cholesterin test
hRf-values
- cholesterin, cholesterin test:
11 - 23
B) Steroid test
- methyltestosterone, steroid test: 20 - 40
- Reichstein S, steroid test: 50 - 70
- hydrocortisone, steroid test: 65 - 85
Typical value determined on an activated plate (120°C, 15 min)
Eluent a) aceton/ water (95/5 v/v)
Eluent b) acetonitrile/ water (70/30 v/v)
Certificates of Analysis (COA)
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