06-284
Anti-Nitrotyrosine Antibody
Upstate®, from rabbit
Synonym(s):
Anti-Nitrotyrosine, Detection Antibody, Nitrotyrosine Detection Antibody
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All Photos(6)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NOS1(4842)
General description
Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others.
Specificity
Recognizes proteins which are nitrated.
Immunogen
Nitrated KLH
Application
- Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected in fixed and permeabilized A-431 cells treated with 5mM peroxynitrite for 15 minutes.
- Immunohistochemistry Applications: A representative lot detected Nitrotyrosine in human colon cancer, human Alzheimer′s brain and human diabetic kidney tissue sections.
- Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Research Sub Category
Neurotransmitters & Receptors
Neurodegenerative Diseases
Neurotransmitters & Receptors
Neurodegenerative Diseases
This rabbit polyclonal Anti-Nitrotyrosine, Cat. No. 06-284 is tested for use in Western Blotting, Immunohistochemistry and Immunocytochemistry, for the detection of Nitrotyrosine.
Quality
routinely evaluated by immunoblot on nitrotyrosine immunoblotting controls (Catalog #12-354)
Target description
Predicted MW for nitrated-BSA: 85 kDa
Physical form
Format: Purified
ImmunoAffinity Purified
Purified Rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4, 0.05% sodium azide, and 10 mg/mL BSA
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Nitric oxide affects the phosphorylation state of microtubule-associated protein 2 (MAP-2) and neurofilament: an immunocytochemical study in the brain of rats and neuronal nitric oxide synthase (nNOS)-knockouts.
Fritz Rothe, Heiko Possel, Gerald Wolf
Nitric Oxide null
IGF-1 overexpression inhibits the development of diabetic cardiomyopathy and angiotensin II-mediated oxidative stress
Kajstura, J., et al
Diabetes, 50, 1414-1424 (2001)
S K Wattanapitayakul et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(2), 271-278 (2000-02-05)
Angiotensin II (ANG II) is a well-established participant in many cardiovascular disorders, but the mechanisms involved are not clear. Vascular cell experiments suggest that ANG II is a potent stimulator of free radicals such as superoxide anion, an agent known
M Miampamba et al.
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 11(3), 193-206 (1999-06-03)
Nitric oxide (NO) has been implicated in the pathogenesis of inflammatory bowel disease since increased NO production is observed in this disease. NO can react with superoxide to generate peroxynitrite which causes and/or exacerbates colitis. Peroxynitrite, in turn, nitrates tyrosine
Reactive nitrogen species contribute to blood-labyrinth barrier disruption in suppurative labyrinthitis complicating experimental pneumococcal meningitis in the rat
Kastenbauer, S., et al
Brain Research, 904, 208-217 (2001)
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