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258385

Sigma-Aldrich

Protoporphyrin IX disodium salt

≥90% (HPLC)

Synonym(s):

3,7,12,17-Tetramethyl-8,13-divinyl-2,18-porphinedipropionic acid disodium salt, Depocolin-S, Ooporphyrin disodium salt

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250 MG
MXP 1,177.00
1 G
MXP 4,009.00
5 G
MXP 12,457.00

MXP 1,177.00


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250 MG
MXP 1,177.00
1 G
MXP 4,009.00
5 G
MXP 12,457.00

About This Item

Empirical Formula (Hill Notation):
C34H32N4Na2O4
CAS Number:
Molecular Weight:
606.62
Beilstein:
5227034
EC Number:
MDL number:
UNSPSC Code:
12352103
PubChem Substance ID:
NACRES:
NA.23

MXP 1,177.00


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Quality Level

Assay

≥90% (HPLC)

form

powder

λmax

406 nm

storage temp.

2-8°C

SMILES string

[Na+].[Na+].Cc1c(CCC([O-])=O)c2cc3[nH]c(cc4nc(cc5[nH]c(cc1n2)c(C=C)c5C)c(C=C)c4C)c(C)c3CCC([O-])=O

InChI

1S/C34H34N4O4.2Na/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;;/h7-8,13-16,36-37H,1-2,9-12H2,3-6H3,(H,39,40)(H,41,42);;/q;2*+1/p-2/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;;

InChI key

JZRNDUPVNXWZDY-HXFTUNQESA-L

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Application

Protoporphyrin disodium salt (PpIX) was used as a dye content for fluorescence spectroscopic characterization in a study. [1] The cytotoxic effect of PpIX on sarcoma 180 solid tumors cells was reported in a study. [2][3]

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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1 of 1

Sonodynamic effects of protoporphyrin IX disodium salt on isolated sarcoma 180 cells
Liu Q, et al.
Ultrasonics, 45(1-4), 56-60 (2006)
Fluorescence spectroscopic characterization of salivary metabolites of oral cancer patients.
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5-Aminolevulinic acid (ALA) is a precursor of heme. ALA is used as a photosensitive substance in photodynamic diagnosis (PDD) and photodynamic therapy (PDT) because heme metabolism is abnormal in tumor cells and a photosensitive metabolite of heme synthesis from ALA
Filippo Piffaretti et al.
Journal of biomedical optics, 17(11), 115007-115007 (2012-12-12)
Tissular oxygen concentration plays a key role during photodynamic therapy (PDT). Therefore, monitoring its local oxygen partial pressure (pO2) may help predict and/or control the outcome of a PDT treatment. The first real-time, in vivo measurements of the pO2 in
Woo Hyung Lee et al.
Chemico-biological interactions, 204(1), 49-57 (2013-04-30)
Porphyrins are used for photodynamic therapy for their light-absorbing properties, and some of them were approved for the treatment of certain types of cancers. Porphyrins prevent activation of hypoxia inducible factor-1α (HIF-1α) by inhibiting heat shock protein 90 (HSP90). This

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Questions

1–2 of 2 Questions  
  1. To study interaction of porphyrin with a protein, how should a solution of this compound be made for flourescence quenching?

    1 answer
    1. This is not an application that is tested internally, but there is evidence in literature (Iwunze MO. The fluorescence study of the quenching of nanoemulsion by protoporphyrin IX (PPIX). Material Sci & Eng. 2022;6(3):97‒99. DOI: 10.15406/mseij.2022.06.00185) that concentrations between 6.45 x 10-7 M to 3.24 x 10-6 M PPIX are effective for quenching. Another author describes the quenching of protein fluorescence by PPIX by using a solution concentration range of 1×10−5 M to 3×10−4 M excited at 280 nm and 1x10−5 M to 3×10−4 M excited at 295 nm (Characteristics of the Protoporphyrin IX Binding Sites on Human Serum Albumin Using Molecular Docking, Molecules 2016, 21(11), 1519).

      Helpful?

  2. What is the water solubility of PPIX-disodium?

    1 answer
    1. This chemical is known to be soluble in chloroform or methanol and slightly soluble in water. The concentration at which this product is solubilized is not determined.

      Helpful?

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