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Key Documents

RAB1271

Sigma-Aldrich

Human Arginase 1 ELISA

for serum, plasma and cell culture supernatants

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2.05 ng/mL
standard curve range: 2.048-500 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... ARG1(383)

General description

The gene encoding arginase 1 (ARG1), spanning 10-15kb on genomic DNA, is localized on human chromosome 6q23.2. It consists of eight exons. The protein consists of 322 amino acids and is expressed in the liver, red blood cells, lactating mammalian glands and the kidney.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached Protocolfor details.

Biochem/physiol Actions

Arginase 1 (ARG1) is a liver enzyme involved in the hydrolysis of arginine to form ornithine and urea. In addition to urea cycle, ARG1 is also implicated in various cellular processes, such as macrophage-mediated cytotoxicity and regulation of immune response. The encoded protein is also involved in allergen challenged lungs, in autoimmune inflammation in the central nervous system and in acute liver injury. Deficiency or mutations in the gene leads to a rare urea cycle disorder, argininemia.

Other Notes

A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Carbon nanotube-based sensing devices for human Arginase-1 detection
S. Baldo
Sensitization of Cancer Cells for Chemo/Immuno/Radio-therapy, 7 (2016)
Five Novel Mutations in ARG1 Gene in Chinese Patients of Argininemia
Tong-Fei Wu MD
Pediatric Neurology (2013)
Fernando Holguin et al.
JCI insight, 4(24) (2019-11-13)
BACKGROUNDThe airways of obese asthmatics have been shown to be NO deficient, and this contributes to airway dysfunction and reduced response to inhaled corticosteroids. In cultured airway epithelial cells, L-citrulline, a precursor of L-arginine recycling and NO formation, has been
Clinical phenotype, biochemical profile, and treatment in 19 patients with arginase 1 deficiency
Martina Huemer
Journal of inherited metabolic disease, 39 (2016)

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