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P7643

Sigma-Aldrich

PIPES dipotassium salt

≥99% (titration)

Synonym(s):

1,4-Piperazinediethanesulfonic acid dipotassium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) dipotassium salt

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About This Item

Empirical Formula (Hill Notation):
C8H16K2N2O6S2
CAS Number:
108321-27-3
Molecular Weight:
378.55
MDL number:
MFCD00069751
UNSPSC Code:
12161700
PubChem Substance ID:
24898738
NACRES:
NA.25

Assay

≥99% (titration)

form

crystalline powder

useful pH range

6.1-7.5

pKa (25 °C)

6.8

solubility

water: 0.25 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

[K+].[K+].[O-]S(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O6S2.2K/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;;/h1-8H2,(H,11,12,13)(H,14,15,16);;/q;2*+1/p-2

InChI key

BJQYFCAMUXGZFN-UHFFFAOYSA-L

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General description

PIPES (1,4-Piperazinediethanesulfonic acid) is a zwitterionic buffer. It is a stable buffer and is used majorly in fixation solutions. PIPES is inert towards enzymatic and non-enzymatic reactions. However, it generates artefacts in transmission electron microscope imaging.

Application

PIPES (1,4-Piperazinediethanesulfonic acid) dipotassium salt has been used as buffer for fluorescence polarization microscopy studies of kinesin-microtubule binding. It is suitable for use as a component of PHEM buffer ([K-PIPES], HEPES, EGTA, and MgSO4) used during rinsing and blocking steps in immunofluorescence imaging of HeLa cells. It is also suitable for use as buffer for purification of tubulin.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dynamic phosphorylation of NudC by Aurora B in cytokinesis
Weiderhold KN
PLoS ONE, 11(4), e0153455-e0153455 (2016)
Use of single molecule fluorescence polarization microscopy to study protein conformation and dynamics of kinesin-microtubule complexes
Benoit MPMH and Sosa H
Single Molecule Analysis, 15(1), 199-216 (2018)
Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation
Nie J, et al.
BMC Developmental Biology, 15(1), 10-10 (2015)
Helical alignment inversion of microtubules in accordance with a structural change in their lattice
Shikinaka K, et al.
Soft Matter, 11(19), 3869-3874 (2015)
Nina Marie Pedersen et al.
The Journal of cell biology, 219(8) (2020-06-02)
Cancer cells break tissue barriers by use of small actin-rich membrane protrusions called invadopodia. Complete invadopodia maturation depends on protrusion outgrowth and the targeted delivery of the matrix metalloproteinase MT1-MMP via endosomal transport by mechanisms that are not known. Here

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