The volume of this product will vary from lot to lot. The minimum concentration is 5 mg/mL, however a survey of recent lots shows an average of 13 mg/mL. A 1mg vial will have an average volume of 77 uL. The exact protein concentration is reported on the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/l9420#product-documentation
L9420
Luciferase from Photinus pyralis (firefly)
recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein
Synonym(s):
Luciferase firefly
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.
Recommended Products
recombinant
expressed in E. coli
Quality Level
Assay
≥98% (SDS-PAGE)
form
buffered aqueous solution
specific activity
≥10×1010 units/mg protein
mol wt
62 kDa
shipped in
dry ice
storage temp.
−20°C
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General description
Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light.[1] The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.[2]
Application
Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.[3]
Unit Definition
One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.
Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.
Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.
Physical form
Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Resp. Sens. 1 - STOT RE 2 Oral
Target Organs
Kidney
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
231.8 °F
Flash Point(C)
111 °C
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