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Merck

IP50

Protein G Immunoprecipitation Kit

sufficient for 50 assays

Synonym(s):

Immunoprecipitation kit

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NACRES:
NA.32
UNSPSC Code:
12352203

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Product Name

Protein G Immunoprecipitation Kit, sufficient for 50 assays

usage

sufficient for 50 assays

technique(s)

immunoprecipitation (IP): suitable

storage temp.

2-8°C

Quality Level

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AA0100ET0300IP0010
technique(s)

immunoprecipitation (IP): suitable

technique(s)

-

technique(s)

-

technique(s)

immunoprecipitation (IP): suitable

usage

sufficient for 50 assays

usage

sufficient for 100 assays

usage

sufficient for 100 assays

usage

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Application

The kit is designed to allow maximal recovery of immunoprecipitates. It provides all the necessary reagents to perform immunoprecipitation from cell extracts of any protein to which a suitable antibody is available. Based on protein G, the kit binds to most commonly used antibodies. In addition, spin columns are provided to enable quick washes without the loss of protein G resin and thus protein yield is maximized.

Features and Benefits

  • Minimal loss of antigen-antibody bound beads during washing.
  • Minimal or no non-specific signals by increasing the stringency of the washing step.

Preparation Note

When preparing reagents, use ultrapure water (17M -cm)

Kit Components Also Available Separately

Product No.
Description
SDS

  • S65465 M Sodium chloride solution 15 mLSDS

  • P3296Protein G Agarose 2 mLSDS

  • 7173610% Sodium dodecyl sulfate solution 1 mLSDS

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Warning

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2

Storage Class

3 - Flammable liquids

wgk

WGK 3


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Patricia Workman et al.
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Journal of bacteriology, 190(5), 1507-1517 (2008-01-01)
In Escherichia coli, YaeT, together with four lipoproteins, YfgL, YfiO, NlpB, and SmpA, forms a complex that is essential for beta-barrel outer membrane protein biogenesis. Data suggest that YfgL and YfiO make direct but independent physical contacts with YaeT. Whereas
Kong-Hung Sze et al.
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Peter J Eastmond
The Plant cell, 19(4), 1376-1387 (2007-04-24)
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Drew Bennion et al.
Molecular microbiology, 77(5), 1153-1171 (2010-07-06)
BamA of Escherichia coli is an essential component of the hetero-oligomeric machinery that mediates β-barrel outer membrane protein (OMP) assembly. The C- and N-termini of BamA fold into trans-membrane β-barrel and five soluble POTRA domains respectively. Detailed characterization of BamA

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