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GE28-9365-45

HiPrep™ Phenyl Fast Flow (high sub) 16/10

Cytiva 28-9365-45

Synonym(s):

Phenyl column

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About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

ligand

phenyl

form

prepacked column

crosslinking

cross-linked

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9365-45

storage condition

(20% Ethanol (or 0.01 M NaOH))

parameter

1.5 bar (22 psi) (Over the Packed Bed During Operation)
2 ml/min - 10 ml/min flow rate
22 psi

technique(s)

liquid chromatography (LC): suitable

bed volume

20 mL

column I.D.

16 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14

working range

3-13

suitability

suitable for bioprocess medium

separation technique

hydrophobic interaction (HIC)

Related Categories

General description

HiPrep™ Phenyl FF (High Sub) 16/10, are prepacked Phenyl Sepharose™ 6 Fast FLow columns ready to use for hydrophobic interaction chromatography (HIC). The columns are designed for initial and intermediate purification steps, removing bulk impurities.

Features and Benefits

  • 20 mL HIC columns provide fast, preparative separation of Proteins and other biomolecules.
  • Provide high capacity, excellent flow properties, and reliable, reproducible separations.
  • Phenyl Sepharose™ 6 Fast FLow is a well-established, standard aromatic HIC medium for capture and intermediate purification requiring Low to medium hydrophobicity.
  • Two levels of substitution help find optimal selectivity and binding capacity for a given application.
  • Compatible with simple pump-based configurations or chromatography system such as ÄKTA® design.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

related product

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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