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F9554

Sigma-Aldrich

Anti-Fatty Acid Synthase antibody produced in rabbit

1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-FAS, Anti-FASN

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 270 kDa

species reactivity

mouse, rat, human (predicted)

concentration

1 mg/mL

technique(s)

western blot: 0.5-1 μg/mL using lysates of mouse myoblast C2 cells
western blot: 0.5-1 μg/mL using lysates of rat hepatocytoma FAO cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FASN(2194)
mouse ... Fasn(14104)
rat ... Fasn(50671)

General description

Fatty acid synthase (FAS, FASN) is encoded by the gene mapped to human chromosome 17q25.3.
Fatty acid synthase (FAS, FASN) is encoded by the gene mapped to human chromosome 17q25.3. In mammals, it is composed of two identical multifunctional polypeptides, in which three catalytic domains in the N-terminal section (β-ketoacyl synthase, malonyl/acetyl tansferase, and dehydrase) are separated by a core region of about 600 residues from four C-terminal domains (enoyl reductase, β-ketoacyl reductase, acyl carrier protein, and thioesterase).

Immunogen

synthetic peptide corresponding to amino acids 1982-1999 of mouse fatty acid synthase, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is conserved in rat and differs from the human sequence by three amino acids.

Application

Anti-Fatty Acid Synthase antibody is suitable for western blot analysis at a concentration of 0.5-1μg/mL using lysates of mouse myoblast C2 cells and rat hepatocytoma FAO cells.
Fatty acid synthase antibody produced in rabbit has been used in immunohistochemistry and western blot analysis.

Biochem/physiol Actions

FAS plays an essential role during embryogenesis and energy homeostasis in adult animals. It also acts as the carbon source for fatty acid synthesis. It has been shown to be overexpressed in various types of human cancer, including prostate, breast, ovary and others. FASN is a multifunctional protein, mainly involved in catalyzing the synthesis of palmitate from acetyl-CoA and malonyl CoA, in the presence of NADPH.

Target description

Fatty Acid Synthase encodes an enzyme that is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C Rufo et al.
The Journal of biological chemistry, 276(24), 21969-21975 (2001-03-30)
Refeeding carbohydrate to fasted rats induces the transcription of genes encoding enzymes of fatty acid biosynthesis, e.g. fatty-acid synthase (FAS). Part of this transcriptional induction is mediated by insulin. An insulin response element has been described for the fatty-acid synthase
Bobby Guillory et al.
Aging cell, 17(1) (2017-10-13)
Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide. NAFLD usually begins as low-grade hepatic steatosis which further progresses in an age-dependent manner to nonalcoholic steatohepatitis (NASH), fibrosis, cirrhosis, and hepatocellular carcinoma in some patients. Ghrelin is
Henriette Kirchner et al.
Molecular metabolism, 5(3), 171-183 (2016-03-16)
Epigenetic modifications contribute to the etiology of type 2 diabetes. We performed genome-wide methylome and transcriptome analysis in liver from severely obese men with or without type 2 diabetes and non-obese men to discover aberrant pathways underlying the development of
Francisco J Asturias et al.
Nature structural & molecular biology, 12(3), 225-232 (2005-02-16)
De novo synthesis of fatty acids in the cytosol of animal cells is carried out by the multifunctional, homodimeric fatty acid synthase (FAS). Cryo-EM analysis of single FAS particles imaged under conditions that limit conformational variability, combined with gold labeling
Fatty acid synthase, a proficient multifunctional enzyme.
S J Wakil
Biochemistry, 28(11), 4523-4530 (1989-05-30)

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