E4643

Heparin-Binding EGF-Like Growth Factor human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

• Synonym(s): hHB-EGF, HB-EGF, Heparin-Binding Epidermal Growth Factor-Like Growth Factor
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00212935
• Biological source: human
• Recombinant: expressed in baculovirus infected Sf21 cells
• Assay: ≥97% (SDS-PAGE and N-terminal analysis)
• Form: lyophilized powder
• Mol wt: 9.5 kDa

Properties

• Product Name: Heparin-Binding EGF-Like Growth Factor human, HB-EGF, recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
• biological source: human
• Quality Segment: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• assay: ≥97% (SDS-PAGE and N-terminal analysis)
• form: lyophilized powder
• potency: ≤3 ng/mL
• quality: endotoxin tested
• mol wt: 9.5 kDa
• packaging: pkg of 50 and 250 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: Q99075
• storage temp.: −20°C

Description

Biochem/physiol Actions

Heparin-binding epidermal growth factor (HB-EGF), like BTC, is a heparin-binding EGF family member. Natural soluble HB-EGF is a 19 kDa glycoprotein of multiple forms due to heterogeneous truncations. Soluble HB-EGF is mitogenic and chemotactic for fibroblasts, keratinocytes, and smooth muscle cells but not capillary endothelial cells. Production of HB-EGF is detected in monocytes, macrophages, and vascular and aortic smooth muscle cells (SMC). HB-EGF may play a role in the pathogenesis of atherosclerosis. It may also be an effector of Jun-induced oncogenic transformation. HB-EGF binds to EGFR with high affinity and to ErbB with moderate affinity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in the EGF-responsive BALB/3T3 cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)