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E2886

Sigma-Aldrich

ExtrAvidin®−Peroxidase

buffered aqueous solution

Synonym(s):

ExtrAvidin HRP

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About This Item

MDL number:
MFCD00163600
UNSPSC Code:
12352203
NACRES:
NA.46

conjugate

peroxidase conjugate

form

buffered aqueous solution

technique(s)

direct ELISA: 1:2,000
dot blot: 1:4,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

shipped in

wet ice

storage temp.

2-8°C

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Specificity

The product is found to be reactive with Anti-Avidin, Catalog Number A5170, and non-reactive with Anti-Streptavidin, Catalog Number S6390, by immunoelectrophoresis.

Application

ExtrAvidin®-Peroxidase is suitable for the following applications:
  • Dot blot at a minimum dilution of 1:4,000
  • Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)
  • Direct ELISA at a minimum dilution of 1:2,000
It has also been used for:
  • Immunoprecipitation and Immunobloting
  • Ra2I solid-phase binding assays
  • Southwestern blotting
  • Western blotting
  • Immunocytochemistry

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

Affinity purified protein

Storage and Stability

Store at 2-8 °C. Do Not Freeze. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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L Y Bourguignon et al.
The Journal of biological chemistry, 272(44), 27913-27918 (1997-11-05)
In this study we have examined the interaction between CD44s (the standard form) and the p185(HER2) proto-oncogene in the ovarian carcinoma cell line. Surface biotinylation followed by wheat germ agglutinin column chromatography and anti-CD44-mediated immunoprecipitation indicate that both CD44s and
S Correia et al.
Molecular human reproduction, 19(9), 581-589 (2013-04-26)
Sperm undergo maturation acquiring progressive motility and the ability to fertilize oocytes through exposure to the components of the epididymal fluid (EF). Although the establishment of a calcium (Ca(2+)) gradient along the epididymis has been described, its direct effects on
Dimer-tetramer transition between solution and crystalline states of streptavidin and avidin mutants.
Pazy Y, et al.
Journal of Bacteriology, 185(14), 4050-4056 (2003)
G Grouard et al.
The Journal of experimental medicine, 185(6), 1101-1111 (1997-03-17)
A subset of CD4+CD11c-CD3- blood cells was recently shown to develop into dendritic cells when cultured with monocyte conditioned medium. Here, we demonstrate that CD4+ CD11c-CD3- cells, isolated from tonsils, correspond to the so-called plasmacytoid T cells, an obscure cell
M C Dieu-Nosjean et al.
The Journal of experimental medicine, 192(5), 705-718 (2000-09-07)
Dendritic cells (DCs) form a network comprising different populations that initiate and differentially regulate immune responses. Langerhans cells (LCs) represent a unique population of DCs colonizing epithelium, and we present here observations suggesting that macrophage inflammatory protein (MIP)-3alpha plays a
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Protocols

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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