SCC210
UFH-001 Human Breast Cancer Cell Line
Human
Synonym(s):
CAIX-positive breast cancer cells, triple negative breast cancer cells
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81
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product name
UFH-001 Human Breast Cancer Cell Line, The UFH-001 triple negative CAIX+ human breast cancer cell line is suitable as a model for studying the mechanisms of triple negative breast cancer progression, metastasis and migration.
biological source
human
technique(s)
cell culture | mammalian: suitable
General description
The UFH-001 triple negative CAIX+ human breast cancer cell line constitutively expresses CAIX under both hypoxic and normoxic conditions, exhibits an aggressive TNBC phenotype in vivo, and supports tumor growth in a mouse xenograft model . The constitutive expression of CAIX in UFH-001 cells facilitates its evaluation as a potential therapeutic target both in vitro and in vivo.
Source:
UFH-001 cells are derived from MCF-10A cells, a spontaneously immortalized breast cancer epithelial cell line originating from breast tissue of a healthy female patient . UFH-001 cells were sorted for CAIX expression by flow cytometry .
Source:
UFH-001 cells are derived from MCF-10A cells, a spontaneously immortalized breast cancer epithelial cell line originating from breast tissue of a healthy female patient . UFH-001 cells were sorted for CAIX expression by flow cytometry .
Triple negative breast cancer (TNBC) accounts for 15-20% of breast cancer cases and is characterized by the absence of estrogen receptor (ER), progesterone receptor (PR), and human EGF receptor 2 (HER2) . TNBC is typically more aggressive and difficult to treat than more common types of breast cancer and is more likely to recur after treatment. Carbonic anhydrase IX (CAIX) catalyzes the reversible hydration of carbon dioxide and is a marker for hypoxic regions of breast tumors. CAIX expression is associated with poor prognosis in TNBC and hormone-resistant breast tumors.
Cell Line Description
Cancer Cells
Application
Research Category
Cancer
Cancer
The UFH-001 triple negative CAIX+ human breast cancer cell line is suitable as a model for studying the mechanisms of triple negative breast cancer progression, metastasis and migration.
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Quality
• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
• Cells are tested negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Storage and Stability
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Zhijuan Chen et al.
Cancer biology & therapy, 19(7), 598-608 (2018-03-22)
Human cell lines are an important resource for research, and are often used as in vitro models of human diseases. In response to the mandate that all cells should be authenticated, we discovered that the MDA-MB-231 cells that were in
H D Soule et al.
Cancer research, 50(18), 6075-6086 (1990-09-25)
Two sublines of a breast epithelial cell culture, MCF-10, derived from human fibrocystic mammary tissue exhibit immortality after extended cultivation in low calcium concentrations (0.03-0.06 mM) and floating transfers in low calcium (MCF-10F), or by trypsin-Versene passages in the customary
Zuzana Sporikova et al.
Clinical breast cancer, 18(5), e841-e850 (2018-08-28)
Triple-negative breast cancer (TNBC) accounts for 15% to 20% of breast cancer cases and is characterized by the absence of estrogen, progesterone, and human epidermal growth factor 2 receptors. Though TNBC is a highly heterogenic and aggressive disease, TNBC patients
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