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MABC570

Sigma-Aldrich

Anti-Sec8 Antibody/Exoc4, clone 2E12

clone 2E12, from mouse

Synonym(s):

Exocyst complex component 4, Exocyst complex component Sec8, rSec8

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

2E12, monoclonal

species reactivity

human, rat, mouse

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... EXOC4(60412)

Related Categories

General description

Sec8/Exoc4 is a component of the exocyst complex which is a multi-protein complex used for docking of exocytic vesicles with fusion sites on the plasma membrane. The exocyst complex is essential for exocytosis which is the process whereby proteins, hormones, transmitters etc. are transported and released to the extracellular environment. The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Sec8/Exoc4 appears to be important for tethering of secretory vesicles to specific regions on the plasma membrane. It seems particularly important in normal development for growth and morphological change by allowing the secretion of matrix metalloproteinases such as MMP2 or MMP9 in discrete locations. Knockdowns exhibit reduced cellular proliferation as well as invasive activities as well. As such Sec8/Exoc4 is important in tumor growth and invasion as well, thus it is also a current target for cancer therapy.

Immunogen

Recombinant protein corresponding to rat Sec8/Exoc4.

Application

Immunocytochemistry Analysis: A 1:50-250 dilution from a representative lot detected Sec8/Exoc4 in HeLa, A431, and NIH/3T3 cells. DyLight® is a registered trademark of Thermo Fisher Scientific.
Western Blotting Analysis: A representative lot detected Sec8/Exoc4 in HeLa cells treated with EXO70 SiRNA (Liu, J., et al. (2007). Molecular Biol. Cell. 18:4483-4492).
Western Blotting Analysis: A representative lot detected Sec8/Exoc4 in B16F1 cells treated with siRNA targeting Sec8 (Zhao, Y., et al. (2013). Dev. Cell. 26:266-278).
Immunocytochemistry Analysis: A representative lot detected Sec8/Exoc4 in HeLa cells transfected with GFP-Exo70 (Liu, J., et al. (2007). Molecular Biol. Cell. 18:4483-4492).
Immunocytochemistry Analysis: A representative lot detected Sec8/Exoc4 in Rat emreyonic hippocampal neurons (Menchon, S.A., et al. (2011). PLOS ONE. 6(9):e24190 1-11).
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-Sec8 Antibody/Exoc4, clone 2E12 is validated for use in Western Blotting and Immunocytochemistry for the detection of Sec8/Exoc4.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Sec8/Exoc4 in 10 µg of HeLa cell lysate.

Target description

~110 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

DyLight is a registered trademark of Pierce Biotechnology, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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