MAB079-1

Anti-S-100 Protein Antibody, clone 15E2E2

clone 15E2E2, Chemicon^®, from mouse

• Synonym(s): Calcium Binding Protein A1/B1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 15E2E2, monoclonal
• species reactivity: bovine, rat, human, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NM_006271.1
• UniProt accession no.: P23297
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... S100A1(6271)

Description

General description

S-100 protein is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. The protein exists in both heterodimeric and homodimeric forms. The two subunits of S-100 protein are the products of separate genes that are differentially expressed by various cells. The beta subunit is present in all S-100 positive cells and tumors. In contrast, the alpha subunit is detectable only in heart, skeletal muscle and brain. In addition to its calcium binding properties, S-100 protein has a high-affinity binding site for zinc and is involved in the regulation of protein phosphorylation in the brain. The protein apparently plays a role in cell differentiation and growth, cytoskeletal structure and function, and has been implicated in neuropathological diseases.

Specificity

This antibody recognizes ~22 kDa (non-reduced) or 11 kDa (reduced) of S100. It reacts with human, rat, mouse, and cow (bovine). Other species are not tested.

Immunogen

Epitope: Recognizes both alpha & beta S100

Purified bovine S-100 protein

Application

Anti-S-100 Protein Antibody, clone 15E2E2 is an antibody against S-100 Protein for use in IH, IH(P), IP & WB.

Immunoprecipitation:
A previous lot of this antibody was used in IP.

Visualization of cellular and subcellular localization of S-100 protein using immunohistochemical techniques.

Immunohistochemistry:
A 1:100-200 dilution of a previous lot was used for paraffin embedded tissue sections.

Optimal working dilutions must be determined by end user.

Research Category
Metabolism

Research Sub Category
Muscle Physiology

Quality

Evaluated by Western Blot on PC12 lysates

Western Blot Analysis:
1:1000 dilution of this antibody detected S100 on 10 µg of PC12 lysate.

Physical form

Format: Purified

The mouse IgG is purified by protein A-affinity chromatography according to isotyping.

This affinity purified antibody is supplied in sterile phosphate buffered saline (pH 7.2) containing antibody stabilizer. Contains 0.01% sodium azide.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Positive Control Tissue: Melanoma

Other Notes

Concentration: 0.5 mg/mL

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable