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Key Documents

07-215

Sigma-Aldrich

Anti-dimethyl-Histone H3 (Arg26) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

H3R26me2, Histone H3 (di methyl R26), H3 histone family, member T, histone 3, H3, histone cluster 3, H3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

vertebrates, Tetrahymena sp., Xenopus, human

technique(s)

multiplexing: suitable
western blot: suitable

NCBI accession no.

NP_003484.1

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

dimethylation (Arg26)

Gene Information

human ... H3C1(8350)

General description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Specificity

Recognizes CARM1 in vitro methylated recombinant Histone H3. The immunizing sequence is conserved from Tetrahymena through human therefore a broad cross reactivity is expected.
Recognizes dimethylated Histone H3 at and around arginine 26, MW ~17 kDa. Possible slight reactivity with monomethyl histone H3 (Arg26).

Immunogen

Epitope: Dimethylated Histone H3 (Arg26)
KLH-conjugated synthetic peptide (ATKAA(di-Me)RKSAPA-C) corresponding to amino acids 21-31 of Human Histone H3.

Application

Anti-dimethyl-Histone H3 (Arg26) Antibody is a rabbit polyclonal antibody for detection of dimethyl-Histone H3 (Arg26) also known as H3R26me2, Histone H3 (di methyl R26) & has been validated in WB, Mplex.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
SNAP I.D. (Western Blotting Technique):
A previous lot of this antibody worked successfully using the SNAP I.D. western blotting device and technique.

Quality

Evaluated by western blot on untreated and CARM1 in vitro methylated recombinant Histone H3.

Western Blot Analysis: 1:8000 dilution of this antibody detected dimethyl-Histone H3 (Arg26) in untreated and CARM1 in vitro methylated recombinant Histone H3.

Target description

17kDa

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl, with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
CARM1 in vitro methylated recombinant Histone H3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Monica Mann et al.
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B Ruthrotha Selvi et al.
The Journal of biological chemistry, 285(10), 7143-7152 (2009-12-22)
Methylation of the arginine residues of histones by methyltransferases has important consequences for chromatin structure and gene regulation; however, the molecular mechanism(s) of methyltransferase regulation is still unclear, as is the biological significance of methylation at particular arginine residues. Here
Ablation of PRMT6 reveals a role as a negative transcriptional regulator of the p53 tumor suppressor.
Neault, M; Mallette, FA; Vogel, G; Michaud-Levesque, J; Richard, S
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CARM1/PRMT4 (for COACTIVATOR-ASSOCIATED ARGININE METHYLTRANSFERASE1/PROTEIN ARGININE METHYLTRANSFERASE4) catalyzes asymmetric dimethylation on arginine (Arg), and its functions in gene regulation is understood only in animal systems. Here, we describe AtPRMT4a and AtPRMT4b as a pair of Arabidopsis (Arabidopsis thaliana) homologs of
Coactivator-associated arginine methyltransferase 1 (CARM1) is a positive regulator of the Cyclin E1 gene.
El Messaoudi, S; Fabbrizio, E; Rodriguez, C; Chuchana, P; Fauquier, L; Cheng, D; Theillet et al.
Proceedings of the National Academy of Sciences of the USA null
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