810603P
Avanti
16:0-10 Doxyl PC
Avanti Research™ - A Croda Brand 810603P, powder
Synonym(s):
1-palmitoyl-2-stearoyl-(10-doxyl)-sn-glycero-3-phosphocholine
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About This Item
Empirical Formula (Hill Notation):
C46H90N2O10P
CAS Number:
Molecular Weight:
862.19
UNSPSC Code:
41141825
NACRES:
NA.25
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Assay
>99% (TLC)
form
powder
packaging
pkg of 1 × 1 mg (810603P-1mg)
manufacturer/tradename
Avanti Research™ - A Croda Brand 810603P
lipid type
ESR probes
phospholipids
shipped in
dry ice
storage temp.
−20°C
General description
Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins and as fluorescent quenchers in lipid bilayer structural studies.
Phosphocholine is considered as a precursor molecule. It is formed during the breakdown of phosphatidylcholine metabolism. 10-Doxyl is a spin label probe.
Application
16:0-10 Doxyl PC may be used:
- in vesicles for quenching PT-(1–46)F4W fluorescence
- as vesicle component for tryptophan based fluorescence emission studies
- to probe the oxygen and Ni[II] ethylenediaminediacetic acid (NiEdda) accessibilities inside liposomes
Biochem/physiol Actions
Phosphatidylcholine (PC) functions as a surfactant within the mucus to form a hydrophobic surface to inhibit bacterial penetrance.
Packaging
5 mL Clear Glass Sealed Ampule (810603P-1mg)
Preparation Note
Product use: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies. For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM , Additional supplemental information.
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
Storage Class Code
11 - Combustible Solids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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