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P6912

Sigma-Aldrich

o-Phenylenediamine dihydrochloride

peroxidase substrate, chromogenic, tablet

Synonym(s):

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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About This Item

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
615-28-1
Molecular Weight:
181.06
Beilstein:
3912045
MDL number:
MFCD00012966
UNSPSC Code:
12352204
PubChem Substance ID:
24898805
NACRES:
NA.83

product name

o-Phenylenediamine dihydrochloride, tablet, 5 mg substrate per tablet

form

tablet

mp

258 °C (dec.) (lit.)

storage temp.

2-8°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

o-Phenylenediamine dihydrochloride has been used as a peroxidase substrate in ELISA (enzyme-linked immunosorbent assay).

Packaging

150 mg total tablet weight

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Signal Word

Warning

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Differential induction of pro- and anti-inflammatory cytokines in whole blood by bacteria: effects of antibiotic treatment.
Frieling JT, et al.
Antimicrobial Agents and Chemotherapy, 41, 1439-1443 (1997)
Role of the carbohydrate recognition domains of mouse galectin-4 in oligosaccharide binding and epitope recognition and expression of galectin-4 and galectin-6 in mouse cells and tissues.
Markova V, et al.
International Journal of Molecular Medicine, 18, 65-76 (2006)
Mona O Mohsen et al.
Allergy, 77(1), 243-257 (2021-09-09)
SARS-CoV-2 caused one of the most devastating pandemics in the recent history of mankind. Due to various countermeasures, including lock-downs, wearing masks, and increased hygiene, the virus has been controlled in some parts of the world. More recently, the availability
C Rebouissou et al.
Blood, 91(12), 4727-4737 (1998-06-17)
Agonist antihuman gp130 transducer monoclonal antibodies (MoAbs) were used in SCID mice to grow myeloma cells whose survival and proliferation is dependent on gp130 transducer activation. The agonist anti-gp130 MoAbs neither bound to murine gp130 nor activated murine cells and
J T Frieling et al.
Cytokine, 6(4), 376-381 (1994-07-01)
measurement of baseline soluble interleukin 6 receptor (sIL-6R) and interleukin 6 (IL-6) levels in biological fluids in non-pathological conditions. Blood and urine were obtained from healthy volunteers. Cerebrospinal fluid (CSF) and synovial fluid (SF) were obtained from patients during spinal
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