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L6007

Sigma-Aldrich

Leucine Aminopeptidase, microsomal from porcine kidney

Type VI-S, lyophilized powder, ≥12 units/mg protein (biuret)

Synonym(s):

Aminopeptidase M, Aminopeptidase N, Cathepsin III, LAP, Leucinamide aminopeptidase, Leucinaminopeptidase

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About This Item

CAS Number:
9054-63-1
Enzyme Commission number:
3.4.11.2 (BRENDA, IUBMB)
EC Number:
232-942-5
MDL number:
MFCD00131501
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Porcine kidney

type

Type VI-S

form

lyophilized powder

specific activity

≥12 units/mg protein (biuret)

mol wt

320 kDa

composition

Protein, 30-70%

storage temp.

−20°C

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General description

Leucine Aminopeptidase (LAP) from porcine is a 320-kDa exopeptidase present in cytosol. It contains zinc in its structure and belongs to the M1 and M17 peptidase families. LAP display bi-lobed structure and has the catalytic domain at the C-terminus and a variable N-terminal domain.

Application

Leucine Aminopeptidase, microsomal from porcine kidney has been used in aminopeptidase N (APN) inhibitor screening assay in adenocarcinoma cell line ES-2 and in the inhibition kinetic studies with APN inhibitor.

Biochem/physiol Actions

Leucine Aminopeptidase (LAP) catalyzes protein hydrolysis at the leucine residue at N-terminal. An altered level of LAP is implicated in tumor proliferation and progression. LAP is a potential biomarker for ovarian epithelial cancer therapy. Porcine LAP hydrolyzes glycine and arginine substrates.

Unit Definition

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.

Physical form

Contains primarily phosphate buffer salts, pH 7.0

inhibitor

Product No.
Description
Pricing

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Novel and highly sensitive fluorescent assay for leucine aminopeptidases
Huang H, et al.
Analytical biochemistry, 391(1), 11-16 (2009)
Refolding of hexameric porcine leucine aminopeptidase using a cationic detergent and dextrin-10 as artificial chaperones
Laslo AC, et al.
Journal of Biotechnology, 140(3-4), 162-168 (2009)
Activity screening and structure-activity relationship of the hit compounds targeting APN/CD13
Wang X, et al.
Fundamental & Clinical Pharmacology, 25(2), 217-228 (2011)
The effect of different species Aminopeptidase N Structure on the activity screening of Aminopeptidase N Inhibitor
Wang X, et al.
Biological & Pharmaceutical Bulletin, 33(10), 1658-1665 (2010)
Leucine aminopeptidases: diversity in structure and function
Matsui M, et al.
Biological Chemistry, 387(12), 1535-1544 (2006)
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