Skip to Content
Merck
All Photos(1)

Key Documents

MAB8566

Sigma-Aldrich

Anti-Poliovirus Blend Antibody, clones 583-G8-G2-A4, 591-B1-H7-D1, and 613-F1-B5-E5

ascites fluid, Chemicon®, from mouse

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

583-G8-G2-A4, monoclonal
591-B1-H7-D1, monoclonal
613-F1-B5-E5, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable

isotype

IgG

shipped in

dry ice

Specificity

Reacts with the Poliovirus 1, 2 and 3.

Application

Detect Poliovirus Blend using this Anti-Poliovirus Blend Antibody, clones 583-G8-G2-A4, 591-B1-H7-D1 & 613-F1-B5-E5 validated for use in IF.
IFA at 1:100-1:400.

Dilute with buffer pH 7.5-8.0 to desired working volume.

Final working dilutions must be determined by end user.

SUGGESTED IFA PROTOCOL:

Substrate slides:

1. Scrape and spot cells onto microscope slide wells.

2. Air dry.

3. Fix in cold acetone for 10 minutes at +4°C.

Stain:

1. Add 15 μl diluted polio antibody.

2. Incubate at +37°C for 30 minutes in a humid chamber.

3. Rinse in PBS for 5 minutes; repeat with fresh PBS.

4. Add 15 μl diluted labeled antibody.

5. Repeat steps 2 and 3.

6. Mount with buffered glycerol.

Storage and Stability

Maintain at -20°C in convenient undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Johanna Marsian et al.
Nature communications, 8(1), 245-245 (2017-08-16)
Poliovirus (PV) is the causative agent of poliomyelitis, a crippling human disease known since antiquity. PV occurs in two distinct antigenic forms, D and C, of which only the D form elicits a robust neutralizing response. Developing a synthetically produced
Mohammad W Bahar et al.
NPJ vaccines, 6(1), 5-5 (2021-01-10)
Global vaccination programs using live-attenuated oral and inactivated polio vaccine (OPV and IPV) have almost eradicated poliovirus (PV) but these vaccines or their production pose significant risk in a polio-free world. Recombinant PV virus-like particles (VLPs), lacking the viral genome

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service