ABS314

Anti-APPL1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): DCC-interacting protein 13-alpha, Dip13-alpha, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, mouse, human
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_036228
• UniProt accession no.: Q9UKG1
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... APPL1(26060)

Description

General description

Adapter protein containing PH domain, PTB domain and leucine zipper motif 1 (APPL1) is also called DCC-interacting protein 13-alpha (Dip13-alpha). APPL1 is necessary for cell proliferation in response to extracellular signals and links Rab5 to nuclear signal transduction. APPL1 is expressed at high levels in heart, ovary, pancreas, and skeletal muscle tissues.

Immunogen

Recombinant protein corresponding to human APPL1.

Application

Detect Adapter protein containing PH domain, PTB domain & leucine zipper motif 1 using this rabbit polyclonal antibody, Anti-APPL1 Antibody validated for use in western blotting, IP, ICC & IHC.

Research Category
Apoptosis & Cancer

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western Blotting Analysis: 0.5 µg/mL of this antibody detected APPL1 in 10 µg of L6 and NIH/3T3 cell lysate.

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated APPL1 in HUVEC cell lysate (Lin, D. C., et al. (2006). Mol Cell Biol. 26(23):8928-8941.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in liver tissues from mice fed with either a standard diet, high fat diet, or a high fat diet with exercise (Marinho, R., et al. (2012). J Cell Physiol. 227(7):2917-2926.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in pancreatic islets and impaired GSIS cells of dietary and obese mice (Cheng, K. K., et al. (2009). Cell Metab. 9(5):417-427.).

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected APPL1 in 10 µg of A431 cell lysate.

Target description

~84 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable