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Benzonase® Nuclease

Purity > 99%, Effective viscosity reduction and removal of nucleic acids from protein solutions

Synonym(s):

Endonuclease from Serratia marcescens

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About This Item

CAS Number:
9025-65-4
Enzyme Commission number:
3.1.30.2 (BRENDA, IUBMB)
MDL number:
MFCD00131010
UNSPSC Code:
12352202
NACRES:
NA.54

product name

Benzonase® Nuclease, Purity > 99%, Effective viscosity reduction and removal of nucleic acids from protein solutions

biological source

Serratia marcescens

Quality Level

300

recombinant

expressed in E. coli

Assay

>99% (SDS-PAGE)

form

buffered aqueous glycerol solution

manufacturer/tradename

Novagen®

storage condition

OK to freeze

concentration

25-29 units/μL

impurities

<0.25 EU/kU Total endotoxin

application(s)

research use

shipped in

wet ice

storage temp.

−20°C

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General description

Benzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. It is effective over a wide range of conditions and possesses an exceptionally high specific activity. The enzyme completely digests nucleic acids to 5′-monophosphateterminated oligonucleotides 2 to 5 bases in length (below the hybridization limit), which is ideal for removal of nucleic acids from recombinant proteins, enabling compliance with FDA guidelines for nucleic acid contamination. The ability of Benzonase to rapidly hydrolyze nucleic acids makes the enzyme an excellent choice for viscosity reduction to reduce processing time and increase yields of protein. For example, the enzyme is compatible with BugBuster and PopCulture Protein Extraction Reagents and can therefore be added along with these reagents to eliminate viscosity and remove nucleic acids from E. coli extracts.

The enzyme consists of two subunits of30 kDa each. It is functional between pH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions).Activity is inhibited by > 150 mM monovalent cations, > 100 mM phosphate, > 100 mMammonium sulfate, or > 100 mM guanidine HCl.

Benzonase Nuclease is available in ultrapure (> 99% by SDS-PAGE) and pure (> 90%) grades at a standard concentration of 25-29 U/µl and at a high concentration (HC) of 250 U/µl. Both preparations are free of detectable protease and have specific activity> 1 × 106 U/mg protein. The > 99% purity grade is tested for endotoxins and contains< 0.25 EU/1000 units. The product is supplied in 50% glycerol. Store at -20&#176;C.



Total endotoxin:< 0.25 EU/1,000 units. Purity: > 99% by SDS-PAGE.
Effective viscosity reduction and removal of nucleic acids from protein solutions

Application

Used for the removal of nucleic acid from protein samples.

Biochem/physiol Actions

Digests native or heat-denatured DNA and RNA.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Erika C Urdaneta et al.
Methods (San Diego, Calif.), 178, 72-82 (2019-10-07)
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Thach H Chu et al.
PLoS pathogens, 16(2), e1008083-e1008083 (2020-02-25)
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American journal of physiology. Heart and circulatory physiology, 319(1), H109-H122 (2020-05-23)
Although cell therapy-mediated cardiac repair offers promise for treatment/management of heart failure, lack of fundamental understanding of how cell therapy works limits its translational potential. In particular, whether reparative cells from failing hearts differ from cells derived from nonfailing hearts
Michael L Wallace et al.
eLife, 9 (2020-02-12)
The lateral habenula (LHb) is an epithalamic brain structure critical for processing and adapting to negative action outcomes. However, despite the importance of LHb to behavior and the clear anatomical and molecular diversity of LHb neurons, the neuron types of
Michiel Boekhout et al.
Molecular cell, 74(5), 1053-1068 (2019-04-21)
Double-strand breaks (DSBs) initiate the homologous recombination that is crucial for meiotic chromosome pairing and segregation. Here, we unveil mouse ANKRD31 as a lynchpin governing multiple aspects of DSB formation. Spermatocytes lacking ANKRD31 have altered DSB locations and fail to
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DNA/RNA Removal in Viral Production

Benzonase®endonuclease efficiently removes nucleic acid contaminants from viral production, crucial for cell and gene therapies and vaccines.

DNA/RNA Removal in Viral Production

Benzonase®endonuclease efficiently removes nucleic acid contaminants from viral production, crucial for cell and gene therapies and vaccines.

DNA/RNA Removal in Viral Production

Benzonase®endonuclease efficiently removes nucleic acid contaminants from viral production, crucial for cell and gene therapies and vaccines.

DNA/RNA Removal in Viral Production

Benzonase®endonuclease efficiently removes nucleic acid contaminants from viral production, crucial for cell and gene therapies and vaccines.

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