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G005890

HPLC Analysis of Paclitaxel and Related Compounds on Ascentis® Express F5

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express F5, 15 cm x 4.6 mm I.D., 5 μm particles (50631-U)
mobile phase: [A] water; [B] acetonitrile; (60:40, A:B)
flow rate: 1.5 mL/min
pressure: 2727 psi (188 bar)
column temp.: 30 °C
detector: UV, 227 nm
injection: 5 μL
sample: taxols 25 μg/mL in 70:15:15, water:methanol:acetonitrile

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Analysis Note

An efficient reverse phase HPLC method for the HPLC analysis of of Paclitaxel (Taxol) and related compounds is developed using the Ascentis® Express F5 5μm Fused Core HPLC column. Paclitaxel is a natural product with antitumor activity. TAXOL (paclitaxel) is obtained via a semi-synthetic process from Taxus baccata.
The developed method can be easily used for the routine quality control of bulk and Parenterals dosage form of Paclitaxel within a short analysis time. This method offers the advantages of higher efficies, reduced backpressure and shorter run times over a traditionally run methods over 5 μm totally porous particle columns.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Baccatin III, ≥95% (HPLC)
    Cephalomannine, ≥97% (HPLC), solid
    Paclitaxel, from Taxus yannanensis, powder

analytical column

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