SRP0166
DNMT3B/DNMT3L Active human
recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)
Synonym(s):
DNA (cytosine-5-)-methyltransferase 3b, ICF, M.HsaIIIB
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About This Item
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biological source
human
recombinant
expressed in baculovirus infected insect cells
Assay
≥90% (SDS-PAGE)
form
aqueous solution
mol wt
27 kDa (DNMT3L)
59 kDa (DNMT3B)
packaging
pkg of 10 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... DNMT3B(1789)
General description
DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) has two subfamilies-DNMT3B and ΔDNMT3B. These are expressed in many transcript variants. Several single-nucleotide polymorphisms are observed within the promoter region of the DNMT3B gene. The gene encoding this protein is localized on human chromosome 20q11.2. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is part of the DNA methyltransferases family. It has a cysteine-rich region containing a novel-type zinc finger domain. The gene encoding it is localized on human chromosome 21q22.3.
Application
Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) is a de novo methyltransferase which targets the un-methylated 5′-C-phosphate-G-3′ (CpG) sites on the gene promoter. It is responsible for crucial epigenetic mechanisms for the regulation of chromosomal stability and for building a new methylation pattern. The protein is associated with promoter hypermethylation in tumorigenesis and lung cancer. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is a non-catalytic accessory factor which is mainly responsible for the stimulation of DNA methylation. It helps the DNA methylation machinery to be positioned on properly chromatinized DNA regions. The protein also helps to provide uniform methylation patterns. It has an important role in spermatogenesis and mutations in the DNMT3L gene might be associated with azoospermia susceptibility.
Physical form
Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCL, 20% glycerol, 4 mM glutathione.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
The CpG Island Encompassing the Promoter and First Exon of Human DNMT3L Gene Is a PcG/TrX Response Element (PRE)
PLoS ONE, 9(4), e93561-e93561 (2014)
DNA methyltransferase 3B -149C/T
polymorphism and the risk of laryngeal
squamous cell carcinoma: a case-control
study
polymorphism and the risk of laryngeal
squamous cell carcinoma: a case-control
study
Genetics and molecular research : GMR, 14(4), 12866-12871 (2015)
Polymorphism of DNA Methyltransferase 3b and Association with Development and Prognosis in Gastric Cancer
PLoS ONE, 10(8), e0134059-e0134059 (2015)
DNMT3L Modulates Significant and Distinct Flanking Sequence Preference for DNA Methylation by DNMT3A and DNMT3B In Vivo
PLoS Genetics, 6(9), e1001106-e1001106 (2010)
Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family.
Genomics, 65(3), 293-298 (2000)
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