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R0525

Sigma-Aldrich

RANK Ligand from mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

Osteoclast differentiation factor (ODF), Osteoprotegerin ligand (OPGL), Receptor activator of NF-κB Ligand (RANKL), TNF-related activation−induced cytokines (TRANCE)

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

mouse

Quality Level

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

10.0-25.0 ng/mL ED50

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 ng/mg
<1 EU/μg

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Tnfsf11(21943)

General description

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or receptor activator of nuclear factor-κB ligand (RANKL) is a type II transmembrane signaling receptor. It has a molecular weight of about 35kDa. The protein possesses an amino-terminal intracellular tail and a carboxy-terminal extracellular region, which has a connecting stalk and a receptor-binding domain.

Application

RANK ligand from mouse has been used to visualize differentiated osteoclasts and to stimulate osteoclast differentiation.
The functions of RANK Ligand include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function induction of osteoclastogenesis, and lymph node organogenesis.The cell surface signaling receptor of RANK Ligand is RANK, which undergoes receptor clustering during signal transduction.

Biochem/physiol Actions

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or RANKL has an important role in osteoclast function and differentiation. It binds to RANK and osteoprotegrin (OPG). Bioactivities of RANKL may be attributed to its binding to these two receptor molecules. Mouse and human RANKL share 85% amino acid sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution of 5 mM sodium phosphate, pH 7.6, and 75 mM NaCl.

Analysis Note

The bioactivity is determined by its ability to induce osteoclast differentiation of RAW 264.7. The expected ED50 for this effect is ≤10 ng/ml.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ningning Jiang et al.
Biological trace element research, 193(1), 226-233 (2019-03-17)
Fluoride accumulates and is toxic to bones. Clinical bone lesions occur in a phased manner, being less severe early in the natural course of skeletal fluorosis. Previous research rarely focused on osteocyte, osteoclast, and osteoblast at the same time, although
p21-/- mice exhibit enhanced bone regeneration after injury
Priyantha Premnath
BMC Musculoskelet. Disord. (2017)
RANKL employs distinct binding modes to engage RANK and the OPG decoy receptor
Christopher A
Structure (2012)
Matrine prevents bone loss in ovariectomized mice by inhibiting RANKL-induced osteoclastogenesis
Xiao Chen
Faseb Journal (2017)
Elizabeth Rendina-Ruedy et al.
Journal of bone and mineral metabolism, 34(4), 380-394 (2015-06-11)
Skeletal fractures are considered a chronic complication of type 2 diabetes mellitus (T2DM), but the etiology of compromised bone quality that develops over time remains uncertain. This study investigated the concurrent alterations in metabolic and skeletal changes in two mouse

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