P5368

Phosphate buffered saline

powder, pH 7.4, BioPerformance Certified, for molecular biology

• Synonym(s): PBS
• MDL number: MFCD00131855
• UNSPSC Code: 12161700
• eCl@ss: 32129211
• NACRES: NA.25

Properties

• product name: Phosphate buffered saline, BioPerformance Certified, pH 7.4
• grade: BioPerformance Certified; for molecular biology
• Quality Level: 200
• form: powder
• storage condition: (Keep container tightly closed in a dry and well-ventilated place.)
• technique(s): immunofluorescence: suitable
• impurities: BioBurden, tested; endotoxin, tested
• color: white
• pH: 7.4
• solubility: water: 9.07-10.0 g/L, clear, colorless
• cation traces: Fe: ≤10 ppm; heavy metals (as Pb): ≤5 ppm
• suitability: suitable for SDS-PAGE; suitable for molecular biology
• application(s): diagnostic assay manufacturing; histology; sample preparation
• foreign activity: DNase, RNase, Protease, none detected
• SMILES string: OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]
• InChI: InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5
• InChI key: LOKCTEFSRHRXRJ-UHFFFAOYSA-I

Description

Application

• Rapid and zero-cost DNA extraction from soft-bodied insects for routine PCR-based applications.: This study used phosphate buffered saline (PBS) to develop a cost-effective and efficient method for DNA extraction from insects, facilitating routine PCR-based applications in molecular biology (Jangra & Ghosh, 2022).


• Injectable amnion hydrogel-mediated delivery of adipose-derived stem cells for osteoarthritis treatment.: PBS was utilized to prepare and deliver adipose-derived stem cells in an injectable hydrogel, showing potential for innovative osteoarthritis treatments (Bhattacharjee et al., 2022).


• Rapid and sensitive detection of SARS-CoV-2 infection using quantitative peptide enrichment LC-MS analysis.: This research employed PBS for sample preparation in a novel LC-MS method for detecting SARS-CoV-2, providing a rapid and sensitive diagnostic tool (Hober et al., 2021).

Packaging

Foil Pouch

Reconstitution

Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); pH 7.4, at 25 °C.

Other Notes

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Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable