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Sigma-Aldrich

Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit

for cellular ATP determination

Synonym(s):

ATP bioluminescence assay kit

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160414
NACRES:
NA.26

storage temp.

−20°C

General description

Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit may be employed for the bioluminescent determination of adenosine 5′-triphosphate (ATP) released from a suspension of viable somatic cells. Estimates of cell concentrations may be calculated if it is assumed that the ATP content per viable cell remains constant. The number of viable somatic cells are selectively counted because, as a cell dies, its ATP is rapidly degraded.

Application

The Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit has been used to determine accurately the level of ATP present in neutrophils isolated from the blood of over 100 human test patients.

Kit Components Only

Product No.
Description

  • FL-AAB Dilution buffer 1 mL/vial

  • FL-AAM Assay mix 1 mL/vial

  • FL-AAS ATP standard 1 mL/vial

  • FL-SAR Somatic cell releasing reagent 1 mL/vial

Pictograms

CorrosionEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

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Sarah Myhill et al.
International journal of clinical and experimental medicine, 2(1), 1-16 (2009-05-14)
This study aims to improve the health of patients suffering from chronic fatigue syndrome (CFS) by interventions based on the biochemistry of the illness, specifically the function of mitochondria in producing ATP (adenosine triphosphate), the energy currency for all body
Linqing Zhen et al.
Reproductive toxicology (Elmsford, N.Y.), 59, 66-79 (2015-11-20)
Hexavalent chromium reportedly induces reproductive toxicity and further inhibits male fertility in mammals. In this study, we investigated the molecular mechanism by which hexavalent chromium affects motility signaling in boar spermatozoa in vitro. The results indicated that Cr(VI) decreased sperm
Li Xinhong et al.
Theriogenology, 116, 71-82 (2018-05-21)
The reproductive efficiency of Meishan pigs is higher than that of Duroc pigs, but the underlying molecular mechanism for this disparity remains unclear. No systematic quantitative proteomics studies, comparing global proteins in Meishan and Duroc boar spermatozoa have been reported.
Y Zhao et al.
Redox biology, 12, 129-138 (2017-02-24)
The signaling of reactive oxygen species (ROS) is essential for the maintenance of normal cellular function. However, whether and how ROS regulate stem cells are unclear. Here, we demonstrate that, in transgenic mice expressing the human manganese superoxide dismutase (MnSOD)
Qicheng Ni et al.
Nature communications, 8, 15755-15755 (2017-06-10)
Diabetes is associated with beta cell mass loss and islet dysfunctions. mTORC1 regulates beta cell survival, proliferation and function in physiological and pathological conditions, such as pregnancy and pancreatectomy. Here we show that deletion of Raptor, which is an essential

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