D4418

3,3′-Diaminobenzidine

SIGMAFAST^™, chromogenic, tablet

• Synonym(s): DAB, DAB tablets, Immunohistology substrate
• CAS Number: 91-95-2
• MDL number: MFCD00007725
• UNSPSC Code: 12352204
• PubChem Substance ID: 24893872
• NACRES: NA.83

Properties

• product name: SIGMAFAST^™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 15 mL
• Quality Level: 200
• form: tablet
• solubility: water: 1 tablet/5 mL
• storage temp.: −20°C
• SMILES string: Nc1ccc(cc1N)-c2ccc(N)c(N)c2
• InChI: 1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2
• InChI key: HSTOKWSFWGCZMH-UHFFFAOYSA-N

Description

Application

Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST^™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Reconstitution

Each tablet set dissolved in 15 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS08,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H302,H315,H318,H335,H341,H350
• Precautionary Statements: P202 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable