ND8/34 Cell Line from mouse

92090904, Mouse x Rat Hybridoma nerve, Neuronal

• UNSPSC Code: 41106514

Properties

• Product Name: ND8/34 Cell Line from mouse, 92090904
• biological source: Mouse x Rat Hybridoma nerve
• description: Mouse neuroblastoma x Rat neurone hybrid
• packaging: tube of 5 μg 92090904-DNA-5UG; pkg of vial of cells 92090904-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Neuronal
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse neuroblastoma x Rat neurone hybrid

Cell Line Description

The hybrid ND8/34 is a subclone of ND8 which was derived after fusion of primary neonatal rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)-derived azaguanine-resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. ND8/34 are electrically excitable to the chemical activator of nociceptive sensory neurons, bradykinin.

Application

Study of neuronal differentiation.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO₂; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

Other Notes

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